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Multiplexed Digital Characterization of Misfolded Protein Oligomers via Solid-State Nanopores.

Published version
Peer-reviewed

Repository DOI


Change log

Authors

Rocchetti, Sara 
Novak, Robert 

Abstract

Misfolded protein oligomers are of central importance in both the diagnosis and treatment of Alzheimer's and Parkinson's diseases. However, accurate high-throughput methods to detect and quantify oligomer populations are still needed. We present here a single-molecule approach for the detection and quantification of oligomeric species. The approach is based on the use of solid-state nanopores and multiplexed DNA barcoding to identify and characterize oligomers from multiple samples. We study α-synuclein oligomers in the presence of several small-molecule inhibitors of α-synuclein aggregation as an illustration of the potential applicability of this method to the development of diagnostic and therapeutic methods for Parkinson's disease.

Description

Publication status: Published

Keywords

Humans, alpha-Synuclein, Nanopores, Parkinson Disease

Journal Title

J Am Chem Soc

Conference Name

Journal ISSN

0002-7863
1520-5126

Volume Title

145

Publisher

American Chemical Society (ACS)
Sponsorship
Engineering and Physical Sciences Research Council (EP/L027151/1)
Engineering and Physical Sciences Research Council (EP/L015978/1)
European Commission Horizon 2020 (H2020) Future and Emerging Technologies (FET) (829067)
Engineering and Physical Sciences Research Council (EP/S022953/1)
European Commission Horizon 2020 (H2020) Research Infrastructures (RI) (861950)
European Commission Horizon 2020 (H2020) ERC (883703)
European Commission Horizon 2020 (H2020) ERC (899538)