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Robertsonian fusion triggers recombination suppression on sex chromosomes in Coleonyx geckos.

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Peer-reviewed

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Authors

Lisachov, Artem 
Tishakova, Katerina 
Romanenko, Svetlana 
Lisachova, Lada 
Davletshina, Guzel 

Abstract

The classical hypothesis proposes that the lack of recombination on sex chromosomes arises due to selection for linkage between a sex-determining locus and sexually antagonistic loci, primarily facilitated by inversions. However, cessation of recombination on sex chromosomes could be attributed also to neutral processes, connected with other chromosome rearrangements or can reflect sex-specific recombination patterns existing already before sex chromosome differentiation. Three Coleonyx gecko species share a complex X1X1X2X2/X1X2Y system of sex chromosomes evolved via a fusion of the Y chromosome with an autosome. We analyzed synaptonemal complexes and sequenced flow-sorted sex chromosomes to investigate the effect of chromosomal rearrangement on recombination and differentiation of these sex chromosomes. The gecko sex chromosomes evolved from syntenic regions that were also co-opted also for sex chromosomes in other reptiles. We showed that in male geckos, recombination is less prevalent in the proximal regions of chromosomes and is even further drastically reduced around the centromere of the neo-Y chromosome. We highlight that pre-existing recombination patterns and Robertsonian fusions can be responsible for the cessation of recombination on sex chromosomes and that such processes can be largely neutral.

Description

Acknowledgements: The research was completed using equipment (materials) of the Core Facilities Centre “Cryobank of cell cultures” Institute of Molecular and Cellular Biology Siberian Branch of Russian Academy of Sciences (Novosibirsk, Russia). The research was funded by the Ministry of Science and Higher Education of the Russian Federation grant number FWNR-2022-0015 (for PB), research grant of the Russian Science Foundation 19-14-00034-P (for KT and SR) and Czech Science Foundation project number 20-27236J (for LK).

Keywords

Female, Animals, Male, Lizards, Sex Chromosomes, Y Chromosome, Cell Movement, Recombination, Genetic

Journal Title

Sci Rep

Conference Name

Journal ISSN

2045-2322
2045-2322

Volume Title

13

Publisher

Springer Science and Business Media LLC
Sponsorship
Russian Science Foundation (19-14-00034-P)
Czech Science Foundation (20-27236J)
Ministry of Science and Higher Education of the Russian Federation (FWNR-2022-0015)