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A direct role for SNX9 in the biogenesis of filopodia.

Published version
Peer-reviewed

Type

Article

Change log

Authors

Jarsch, Iris K 
Nuccitelli, Annalisa 
Mason, Julia 
Shimo, Hanae 

Abstract

Filopodia are finger-like actin-rich protrusions that extend from the cell surface and are important for cell-cell communication and pathogen internalization. The small size and transient nature of filopodia combined with shared usage of actin regulators within cells confounds attempts to identify filopodial proteins. Here, we used phage display phenotypic screening to isolate antibodies that alter the actin morphology of filopodia-like structures (FLS) in vitro. We found that all of the antibodies that cause shorter FLS interact with SNX9, an actin regulator that binds phosphoinositides during endocytosis and at invadopodia. In cells, we discover SNX9 at specialized filopodia in Xenopus development and that SNX9 is an endogenous component of filopodia that are hijacked by Chlamydia entry. We show the use of antibody technology to identify proteins used in filopodia-like structures, and a role for SNX9 in filopodia.

Description

Keywords

Journal Title

The Journal of cell biology

Conference Name

Journal ISSN

0021-9525

Volume Title

219

Publisher

Sponsorship
Wellcome Trust (105602/Z/14/Z, 108467/Z/15/Z, 092096)
European Research Council (281971)