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Chemical Annealing Restructures RNA for Nanopore Detection

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paragon-plus: 5978667  ORCID logo
Earle, Max K.; paragon-plus: 6262842 
paragon-plus: 1045145 


RNA is a key biochemical marker, yet its chemical instability and complex secondary structure hamper its integration into DNA nanotechnology-based sensing platforms. Relying on the denaturation of the native RNA structure using urea, we show that restructured DNA/RNA hybrids can readily be prepared at room temperature. Using solid-state nanopore sensing, we demonstrate that the structures of our DNA/RNA hybrids conform to the design at the single-molecule level. Employing this chemical annealing procedure, we mitigate RNA self-cleavage, enabling the direct detection of restructured RNA molecules for biosensing applications.


Publication status: Published


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American Chemical Society
964995 , H2020 Future and Emerging Technologies (DNA)
UK Research and Innovation (EP/X023311/1)
University of Cambridge (NA)