Repository logo
 

Chemical Annealing Restructures RNA for Nanopore Detection

Published version
Peer-reviewed

Repository DOI


Change log

Authors

paragon-plus: 5978667  ORCID logo  https://orcid.org/0000-0002-3634-4580
Earle, Max K.; paragon-plus: 6262842 
paragon-plus: 1045145 

Abstract

RNA is a key biochemical marker, yet its chemical instability and complex secondary structure hamper its integration into DNA nanotechnology-based sensing platforms. Relying on the denaturation of the native RNA structure using urea, we show that restructured DNA/RNA hybrids can readily be prepared at room temperature. Using solid-state nanopore sensing, we demonstrate that the structures of our DNA/RNA hybrids conform to the design at the single-molecule level. Employing this chemical annealing procedure, we mitigate RNA self-cleavage, enabling the direct detection of restructured RNA molecules for biosensing applications.

Description

Publication status: Published

Keywords

Is Part Of

Publisher

American Chemical Society
Sponsorship
964995 , H2020 Future and Emerging Technologies (DNA)
UK Research and Innovation (EP/X023311/1)
University of Cambridge (NA)