Optogenetic Control of Subcellular Protein Location and Signaling in Vertebrate Embryos.
Published version
Peer-reviewed
Repository URI
Repository DOI
Change log
Authors
Buckley, Clare E
Abstract
This chapter describes the use of optogenetic heterodimerization in single cells within whole-vertebrate embryos. This method allows the use of light to reversibly bind together an "anchor" protein and a "bait" protein. Proteins can therefore be directed to specific subcellular compartments, altering biological processes such as cell polarity and signaling. I detail methods for achieving transient expression of fusion proteins encoding the phytochrome heterodimerization system in early zebrafish embryos (Buckley et al., Dev Cell 36(1):117-126, 2016) and describe the imaging parameters used to achieve subcellular light patterning.
Description
Title
Optogenetic Control of Subcellular Protein Location and Signaling in Vertebrate Embryos.
Keywords
Heterodimerization, Optogenetics, Phytochrome, Polarity, Signaling, Zebrafish, Animals, Embryonic Development, Gene Expression, Microinjections, Optogenetics, Plasmids, Protein Transport, RNA, Messenger, Signal Transduction, Transgenes, Vertebrates, Zebrafish
Is Part Of
Methods in Molecular Biology
Book type
Publisher
Springer New York
Publisher DOI
ISBN
978-1-4939-9008-5
Sponsorship
Wellcome Trust (208758/Z/17/Z)