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bicoid mRNA localises to the Drosophila oocyte anterior by random Dynein-mediated transport and anchoring.

Published version
Peer-reviewed

Type

Article

Change log

Authors

Belaya, Katsiaryna 
Nashchekin, Dmitry 
Sirinakis, George 

Abstract

bicoid mRNA localises to the Drosophila oocyte anterior from stage 9 of oogenesis onwards to provide a local source for Bicoid protein for embryonic patterning. Live imaging at stage 9 reveals that bicoid mRNA particles undergo rapid Dynein-dependent movements near the oocyte anterior, but with no directional bias. Furthermore, bicoid mRNA localises normally in shot2A2, which abolishes the polarised microtubule organisation. FRAP and photo-conversion experiments demonstrate that the RNA is stably anchored at the anterior, independently of microtubules. Thus, bicoid mRNA is localised by random active transport and anterior anchoring. Super-resolution imaging reveals that bicoid mRNA forms 110-120 nm particles with variable RNA content, but constant size. These particles appear to be well-defined structures that package the RNA for transport and anchoring.

Description

Keywords

D. melanogaster, axis formation, bicoid, cell biology, developmental biology, dynein, mRNA localisation, microtubules, stem cells, Animals, Biological Transport, Active, Drosophila, Drosophila Proteins, Dyneins, Homeodomain Proteins, Oocytes, RNA, Messenger, Ribonucleoproteins, Trans-Activators

Journal Title

Elife

Conference Name

Journal ISSN

2050-084X
2050-084X

Volume Title

5

Publisher

eLife Sciences Publications, Ltd
Sponsorship
Biotechnology and Biological Sciences Research Council (BB/F010303/1)
Wellcome Trust (092096/Z/10/Z)
Wellcome Trust (080007/Z/06/Z)
European Commission (236621)
Cancer Research Uk (None)