Plasmodium falciparum GBP2 Is a Telomere-Associated Protein That Binds to G-Quadruplex DNA and RNA


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Authors
Edwards-Smallbone, James 
Jensen, Anders L 
Roberts, Lydia E 
Totañes, Francis Isidore G 
Hart, Sarah R 
Abstract

jats:pIn the early-diverging protozoan parasite jats:italicPlasmodium</jats:italic>, few telomere-binding proteins have been identified and several are unique. jats:italicPlasmodium</jats:italic> telomeres, like those of most eukaryotes, contain guanine-rich repeats that can form G-quadruplex structures. In model systems, quadruplex-binding drugs can disrupt telomere maintenance and some quadruplex-binding drugs are potent anti-plasmodial agents. Therefore, telomere-interacting and quadruplex-interacting proteins may offer new targets for anti-malarial therapy. Here, we report that jats:italicP. falciparum</jats:italic> GBP2 is such a protein. It was identified jats:italicvia</jats:italic> ‘Proteomics of Isolated Chromatin fragments’, applied here for the first time in jats:italicPlasmodium</jats:italic>. jats:italicIn vitro</jats:italic>, jats:italicPf</jats:italic>GBP2 binds specifically to G-rich telomere repeats in quadruplex form and it can also bind to G-rich RNA. jats:italicIn vivo</jats:italic>, jats:italicPf</jats:italic>GBP2 partially colocalises with the known telomeric protein HP1 but is also found in the cytoplasm, probably due to its affinity for RNA. Consistently, its interactome includes numerous RNA-associated proteins. jats:italicPf</jats:italic>GBP2 is evidently a multifunctional DNA/RNA-binding factor in jats:italicPlasmodium</jats:italic>.</jats:p>

Description
Keywords
Cellular and Infection Microbiology, Plasmodium, malaria, G-quadruplex, telomere, proteomics of isolated chromatin fragments, GBP2
Journal Title
Frontiers in Cellular and Infection Microbiology
Conference Name
Journal ISSN
2235-2988
Volume Title
12
Publisher
Frontiers Media SA