Estimating telomere length from whole genome sequence data.
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Peer-reviewed
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Authors
Aviv, Abraham
Spector, Tim
Durbin, Richard
Abstract
Telomeres play a key role in replicative ageing and undergo age-dependent attrition in vivo. Here, we report a novel method, TelSeq, to measure average telomere length from whole genome or exome shotgun sequence data. In 260 leukocyte samples, we show that TelSeq results correlate with Southern blot measurements of the mean length of terminal restriction fragments (mTRFs) and display age-dependent attrition comparably well as mTRFs.
Description
Keywords
Adult, Aged, Base Sequence, Cross-Sectional Studies, Exome, Genome, Human, Humans, Leukocytes, Middle Aged, Models, Genetic, Repetitive Sequences, Nucleic Acid, Sequence Analysis, DNA, Software, Telomere, Telomere Homeostasis
Journal Title
Nucleic Acids Res
Conference Name
Journal ISSN
0305-1048
1362-4962
1362-4962
Volume Title
42
Publisher
Oxford University Press (OUP)
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Sponsorship
Medical Research Council (G0600717)
Medical Research Council (MC_UU_12012/5/B)
Wellcome Trust (100140/Z/12/Z)
Wellcome Trust (091310/Z/10/Z)
Medical Research Council (G0600717/1)
Medical Research Council (MC_UU_12012/5/B)
Wellcome Trust (100140/Z/12/Z)
Wellcome Trust (091310/Z/10/Z)
Medical Research Council (G0600717/1)