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Schistosoma mansoni schistosomula antigens induce Th1/Pro-inflammatory cytokine responses.

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cam.issuedOnline2018-10-21
dc.contributor.authorEgesa, Moses
dc.contributor.authorLubyayi, Lawrence
dc.contributor.authorTukahebwa, Edridah M
dc.contributor.authorBagaya, Bernard S
dc.contributor.authorChalmers, Iain W
dc.contributor.authorWilson, Shona
dc.contributor.authorHokke, Cornelis H
dc.contributor.authorHoffmann, Karl F
dc.contributor.authorDunne, David W
dc.contributor.authorYazdanbakhsh, Maria
dc.contributor.authorLabuda, Lucja A
dc.contributor.authorCose, Stephen
dc.contributor.orcidCose, Stephen [0000-0002-5156-037X]
dc.date.accessioned2018-11-23T00:33:10Z
dc.date.available2018-11-23T00:33:10Z
dc.date.issued2018-12
dc.description.abstractLarvae of Schistosoma (schistosomula) are highly susceptible to host immune responses and are attractive prophylactic vaccine targets, although cellular immune responses against schistosomula antigens in endemic human populations are not well characterized. We collected blood and stool from 54 Schistosoma mansoni-infected Ugandans, isolated peripheral blood mononuclear cells and stimulated them for 24 hours with schistosome adult worm and soluble egg antigens (AWA and SEA), along with schistosomula recombinant proteins rSmKK7, Lymphocyte Antigen 6 isoforms (rSmLy6A and rSmLy6B), tetraspanin isoforms (rSmTSP6 and rSmTSP7). Cytokines, chemokines and growth factors were measured in the culture supernatants using a multiplex luminex assay, and infection intensity was determined before and at 1 year after praziquantel (PZQ) treatment using the Kato-Katz method. Cellular responses were grouped and the relationship between groups of correlated cellular responses and infection intensity before and after PZQ treatment was investigated. AWA and SEA induced mainly Th2 responses. In contrast, rSmLy6B, rSmTSP6 and rSmTSP7 induced Th1/pro-inflammatory responses. While recombinant antigens rSmKK7 and rSmLy6A did not induce a Th1/pro-inflammatory response, they had an association with pre-treatment infection intensity after adjusting for age and sex. Testing more schistosomula antigens using this approach could provide immune-epidemiology identifiers necessary for prioritizing next generation schistosomiasis vaccine candidates.
dc.format.mediumPrint-Electronic
dc.identifier.doi10.17863/CAM.33192
dc.identifier.eissn1365-3024
dc.identifier.issn0141-9838
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/285848
dc.languageeng
dc.language.isoeng
dc.publisherWiley
dc.publisher.urlhttp://dx.doi.org/10.1111/pim.12592
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectSchistosoma mansoni
dc.subjectTh1/pro-inflammatory
dc.subjectcytokines
dc.subjectschistosomula
dc.subjectvaccine
dc.subjectAnimals
dc.subjectAnthelmintics
dc.subjectAntigens, Helminth
dc.subjectCytokines
dc.subjectFemale
dc.subjectHumans
dc.subjectImmunity, Cellular
dc.subjectLarva
dc.subjectLeukocytes, Mononuclear
dc.subjectMale
dc.subjectPraziquantel
dc.subjectSchistosoma mansoni
dc.subjectSchistosomiasis mansoni
dc.subjectTh1 Cells
dc.titleSchistosoma mansoni schistosomula antigens induce Th1/Pro-inflammatory cytokine responses.
dc.typeArticle
dcterms.dateAccepted2018-09-04
prism.issueIdentifier12
prism.publicationDate2018
prism.publicationNameParasite Immunol
prism.startingPagee12592
prism.volume40
pubs.funder-project-idEuropean Commission (242107)
pubs.funder-project-idWellcome Trust (107743/Z/15/Z)
rioxxterms.licenseref.startdate2018-12
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.typeJournal Article/Review
rioxxterms.versionVoR
rioxxterms.versionofrecord10.1111/pim.12592

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