An hiPSC-Derived In Vitro Model of the Blood-Brain Barrier.
Accepted version
Peer-reviewed
Repository URI
Repository DOI
Change log
Authors
Abstract
Human induced pluripotent stem cells (hiPSC) offer a tractable system to model the blood-brain barrier (BBB). Here we detail the assembly of a triple co-culture hiPSC-BBB model, using hiPSC-derived brain microvascular endothelial cells (BMEC), astrocytes, and mural cells (MC). Transendothelial electrical resistance (TEER) and sodium fluorescein (NaFl) permeability can be used to test the barrier properties. The model has applications in studying BBB-related pathology and for drug screening.
Description
Is Part Of
The Blood-Brain Barrier
Book type
Edited volume
Publisher
Springer Nature
Publisher DOI
ISBN
978-1-0716-2291-9
Rights and licensing
Except where otherwised noted, this item's license is described as All Rights Reserved