An hiPSC-Derived In Vitro Model of the Blood-Brain Barrier.
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Peer-reviewed
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Abstract
Human induced pluripotent stem cells (hiPSC) offer a tractable system to model the blood-brain barrier (BBB). Here we detail the assembly of a triple co-culture hiPSC-BBB model, using hiPSC-derived brain microvascular endothelial cells (BMEC), astrocytes, and mural cells (MC). Transendothelial electrical resistance (TEER) and sodium fluorescein (NaFl) permeability can be used to test the barrier properties. The model has applications in studying BBB-related pathology and for drug screening.
Description
Title
An hiPSC-Derived In Vitro Model of the Blood-Brain Barrier.
Keywords
Astrocytes, Blood-Brain Barrier, Cell Differentiation, Cells, Cultured, Coculture Techniques, Endothelial Cells, Humans, Induced Pluripotent Stem Cells, Blood–brain barrier, Co-culture, hiPSC, TEER, Transwell®
Is Part Of
The Blood-Brain Barrier
Book type
Edited volume
Publisher
Springer Nature