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Identification of GOLPH3 Partners in Drosophila Unveils Potential Novel Roles in Tumorigenesis and Neural Disorders.

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dc.contributor.authorSechi, Stefano
dc.contributor.authorKarimpour-Ghahnavieh, Angela
dc.contributor.authorFrappaolo, Anna
dc.contributor.authorDi Francesco, Laura
dc.contributor.authorPiergentili, Roberto
dc.contributor.authorSchininà, Eugenia
dc.contributor.authorD'Avino, Pier Paolo
dc.contributor.authorGiansanti, Maria Grazia
dc.contributor.orcidFrappaolo, Anna [0000-0002-3270-4139]
dc.contributor.orcidPiergentili, Roberto [0000-0001-7584-2171]
dc.contributor.orcidD'Avino, Pier Paolo [0000-0002-4773-6950]
dc.contributor.orcidGiansanti, Maria Grazia [0000-0002-6753-7262]
dc.date.accessioned2021-10-30T01:13:36Z
dc.date.available2021-10-30T01:13:36Z
dc.date.issued2021-09-06
dc.date.updated2021-10-30T01:13:36Z
dc.description.abstractGolgi phosphoprotein 3 (GOLPH3) is a highly conserved peripheral membrane protein localized to the Golgi apparatus and the cytosol. GOLPH3 binding to Golgi membranes depends on phosphatidylinositol 4-phosphate [PI(4)P] and regulates Golgi architecture and vesicle trafficking. GOLPH3 overexpression has been correlated with poor prognosis in several cancers, but the molecular mechanisms that link GOLPH3 to malignant transformation are poorly understood. We recently showed that PI(4)P-GOLPH3 couples membrane trafficking with contractile ring assembly during cytokinesis in dividing <i>Drosophila</i> spermatocytes. Here, we use affinity purification coupled with mass spectrometry (AP-MS) to identify the protein-protein interaction network (interactome) of <i>Drosophila</i> GOLPH3 in testes. Analysis of the GOLPH3 interactome revealed enrichment for proteins involved in vesicle-mediated trafficking, cell proliferation and cytoskeleton dynamics. In particular, we found that dGOLPH3 interacts with the <i>Drosophila</i> orthologs of Fragile X mental retardation protein and Ataxin-2, suggesting a potential role in the pathophysiology of disorders of the nervous system. Our findings suggest novel molecular targets associated with GOLPH3 that might be relevant for therapeutic intervention in cancers and other human diseases.
dc.identifier.doi10.17863/CAM.77541
dc.identifier.issn2073-4409
dc.identifier.otherPMC8468827
dc.identifier.other34571985
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/330097
dc.languageeng
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.sourceessn: 2073-4409
dc.sourcenlmid: 101600052
dc.subjectSpermatogenesis
dc.subjectDrosophila
dc.subjectCell cycle
dc.subjectFMRP
dc.subjectGolgi
dc.subjectGolph3
dc.subjectMale Meiosis
dc.titleIdentification of GOLPH3 Partners in <i>Drosophila</i> Unveils Potential Novel Roles in Tumorigenesis and Neural Disorders.
dc.typeArticle
prism.issueIdentifier9
prism.publicationNameCells
prism.volume10
pubs.funder-project-idRoyal Society International Exchanges Award (2016/R2 International Exchanges IE160510)
pubs.funder-project-idFondazione Italiana per la Ricerca sul Cancro (id. 19686)
pubs.funder-project-idAssociazione Italiana per la Ricerca sul Cancro (IG2017 Id.20779)
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0/
rioxxterms.versionVoR
rioxxterms.versionofrecord10.3390/cells10092336

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