Repository logo
 

Zebrafish neuromesodermal progenitors undergo a critical state transition in vivo.

Published version
Peer-reviewed

Type

Article

Change log

Authors

Toh, Kane 
Saunders, Dillan 
Verd, Berta 
Steventon, Benjamin  ORCID logo  https://orcid.org/0000-0001-7838-839X

Abstract

The transition state model of cell differentiation proposes that a transient window of gene expression stochasticity precedes entry into a differentiated state. Here, we assess this theoretical model in zebrafish neuromesodermal progenitors (NMps) in vivo during late somitogenesis stages. We observed an increase in gene expression variability at the 24 somite stage (24ss) before their differentiation into spinal cord and paraxial mesoderm. Analysis of a published 18ss scRNA-seq dataset showed that the NMp population is noisier than its derivatives. By building in silico composite gene expression maps from image data, we assigned an 'NM index' to in silico NMps based on the expression of neural and mesodermal markers and demonstrated that cell population heterogeneity peaked at 24ss. Further examination revealed cells with gene expression profiles incongruent with their prospective fate. Taken together, our work supports the transition state model within an endogenous cell fate decision making event.

Description

Keywords

Bioinformatics, Biological sciences, Developmental biology

Journal Title

iScience

Conference Name

Journal ISSN

2589-0042
2589-0042

Volume Title

25

Publisher

Elsevier BV
Sponsorship
Wellcome Trust (109408/Z/15/Z)