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dc.contributor.authorPestana, Den
dc.contributor.authorTeixeira, Den
dc.contributor.authorMeireles, Men
dc.contributor.authorMarques, Cen
dc.contributor.authorNorberto, Sen
dc.contributor.authorSá, Cen
dc.contributor.authorFernandes, VCen
dc.contributor.authorCorreia-Sá, Len
dc.contributor.authorFaria, Aen
dc.contributor.authorGuardão, Len
dc.contributor.authorGuimarães, JTen
dc.contributor.authorCooper, Wendyen
dc.contributor.authorSandovici, Ionelen
dc.contributor.authorDomingues, VFen
dc.contributor.authorDelerue-Matos, Cen
dc.contributor.authorMonteiro, Ren
dc.contributor.authorConstância, Men
dc.contributor.authorCalhau, Cen
dc.date.accessioned2017-08-25T10:12:10Z
dc.date.available2017-08-25T10:12:10Z
dc.identifier.issn2045-2322
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/266862
dc.description.abstractEndocrine-disrupting chemicals such as p,p'-dichlorodiphenyldichloroethylene (p,p'-DDE), are bioaccumulated in the adipose tissue (AT) and have been implicated in the obesity and diabetes epidemic. Thus, it is hypothesized that p,p'-DDE exposure could aggravate the harm of an obesogenic context. We explored the effects of 12 weeks exposure in male Wistar rats' metabolism and AT biology, assessing a range of metabolic, biochemical and histological parameters. p,p'-DDE -treatment exacerbated several of the metabolic syndrome-accompanying features induced by high-fat diet (HF), such as dyslipidaemia, glucose intolerance and hypertension. A transcriptome analysis comparing mesenteric visceral AT (vAT) of HF and HF/DDE groups revealed a decrease in expression of nervous system and tissue development-related genes, with special relevance for the neuropeptide galanin that also revealed DNA methylation changes at its promoter region. Additionally, we observed an increase in transcription of dipeptidylpeptidase 4, as well as a plasmatic increase of the pro-inflammatory cytokine IL-1β. Our results suggest that p,p'-DDE impairs vAT normal function and effectively decreases the dynamic response to energy surplus. We conclude that p,p'-DDE does not merely accumulate in fat, but may contribute significantly to the development of metabolic dysfunction and inflammation. Our findings reinforce their recognition as metabolism disrupting chemicals, even in non-obesogenic contexts.
dc.description.sponsorshipThis article was supported by ERDF (European Regional Development Fund) through the operation POCI-01-0145-FEDER-007746 funded by the Programa Operacional Competitividade e Internacionalização – COMPETE2020 and by National Funds through FCT - Fundação para a Ciência e a Tecnologia within CINTESIS, R&D Unit (reference UID/IC/4255/2013); PEst-OE/SAU/UI0038/2011; SFRH/BPD/109158/2015; SFRH/BD/46640/2008, SFRH/BD/64691/2009, SFRH/BD/78367/2011, SFRH/BD/93073/2013, SFRH/BPD/109153/2015, SFRH/BD/47200/2008, SFRH/BPD/75294/2010; and SFRH/BPD/40110/2007.
dc.languageengen
dc.language.isoenen
dc.publisherNature Publishing Group
dc.rightsAttribution 4.0 International*
dc.rightsAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en
dc.titleAdipose tissue dysfunction as a central mechanism leading to dysmetabolic obesity triggered by chronic exposure to p,p'-DDEen
dc.typeArticle
prism.issueIdentifier1en
prism.number2738en
prism.publicationNameScientific Reportsen
prism.volume7en
dc.identifier.doi10.17863/CAM.12928
dcterms.dateAccepted2017-04-19en
rioxxterms.versionofrecord10.1038/s41598-017-02885-9en
rioxxterms.versionVoRen
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/en
rioxxterms.licenseref.startdate2017-04-19en
dc.contributor.orcidCooper, Wendy [0000-0003-3416-9982]
dc.contributor.orcidSandovici, Ionel [0000-0001-5674-4269]
dc.identifier.eissn2045-2322
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idMRC (MR/J001562/1)
pubs.funder-project-idMRC (MC_UU_12012/5)
cam.issuedOnline2017-06-01en


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International