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dc.contributor.authorRaiber, Eun-Angen
dc.contributor.authorHardisty, Robynen
dc.contributor.authorvan Delft, Pieteren
dc.contributor.authorBalasubramanian, Shankaren
dc.date.accessioned2017-09-29T16:40:10Z
dc.date.available2017-09-29T16:40:10Z
dc.identifier.issn2397-3358
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/267460
dc.description.abstractChemically modified bases exist naturally in genomic DNA. Research into these bases has been invigorated by the discovery of several modified bases in the mammalian genome, in particular 5-methylcytosine and its oxidized derivatives, such as 5-(hydroxymethyl)cytosine and 5-formylcytosine, as well as the enzymes that form and process them, such as the DNA methyltransferases and the ten-eleven translocation enzymes. In this Review, we provide an overview of natural modified bases that have been reported in DNA, our current knowledge of their roles, and the techniques that have enabled us to probe their functions. Analytical methods have been invaluable in helping to advance this field. For example, chemical and enzymatic methods have provided the means to detect and decode modified bases, giving rise to an expanding array of sequencing approaches. Advanced liquid chromatography and tandem mass spectrometry have provided the means to detect and quantify modified bases with very high sensitivity, increasing the prospect of discovering unknown modifications. It is already evident that natural modified DNA bases and their associated enzymology are of fundamental importance to normal biology and to disease. The next decade promises to yield more insights, discoveries and applications from this burgeoning field of research.
dc.description.sponsorshipThe Balasubramanian laboratory is supported by core funding from Cancer Research UK (C14303/A17197). S.B. is a senior investigator of the Wellcome Trust (Grant No. 099232/z/12/z).
dc.publisherNature Publishing Group
dc.subjectDNAen
dc.subjectNucleic acidsen
dc.titleMapping and elucidating the function of modified bases in DNAen
dc.typeArticle
prism.number0069en
prism.publicationNameNature Reviews Chemistryen
prism.volume1en
dc.identifier.doi10.17863/CAM.12230
dcterms.dateAccepted2017-07-27en
rioxxterms.versionofrecord10.1038/s41570-017-0069en
rioxxterms.versionAM*
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2017-07-27en
dc.contributor.orcidvan Delft, Pieter [0000-0002-4726-9467]
dc.contributor.orcidBalasubramanian, Shankar [0000-0002-0281-5815]
dc.identifier.eissn2397-3358
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idWellcome Trust (099232/Z/12/Z)
pubs.funder-project-idCancer Research UK (18618)
cam.issuedOnline2017-09-06en
rioxxterms.freetoread.startdate2018-03-06


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