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dc.contributor.authorZhao, Jing
dc.contributor.authorMitrofan, Claudia-Gabriela
dc.contributor.authorAppleby, Sarah L.
dc.contributor.authorMorrell, Nicholas W.
dc.contributor.authorLever, Andrew M. L.
dc.date.accessioned2017-10-03T07:47:07Z
dc.date.available2017-10-03T07:47:07Z
dc.date.issued2016-6-16
dc.identifier.citationJing Zhao, Claudia-Gabriela Mitrofan, Sarah L. Appleby, Nicholas W. Morrell, and Andrew M. L. Lever, “Disrupted Endothelial Cell Layer and Exposed Extracellular Matrix Proteins Promote Capture of Late Outgrowth Endothelial Progenitor Cells,” Stem Cells International, vol. 2016, Article ID 1406304, 13 pages, 2016. doi:10.1155/2016/1406304
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/267663
dc.description.abstractLate outgrowth endothelial progenitor cells (LO-EPC) possess a high proliferative potential, differentiate into vascular endothelial cells (EC), and form networks, suggesting they play a role in vascular repair. However, due to their scarcity in the circulation there is a requirement for ex vivo expansion before they could provide a practical cell therapy and it is currently unclear if they would home and engraft to an injury site. Using an in vitro flow system we studied LO-EPC under simulated injury conditions including EC activation, ischaemia, disrupted EC integrity, and exposed basement membrane. Perfused LO-EPC adhered to discontinuous EC paracellularly at junctional regions between adjacent cells under shear stress 0.7 dyn/cm2. The interaction was not adhesion molecule-dependent and not enhanced by EC activation. LO-EPC expressed high levels of the VE-Cadherin which may explain these findings. Ischaemia reperfusion injury decreased the interaction with LO-EPC due to cell retraction. LO-EPC interacted with exposed extracellular matrix (ECM) proteins, fibronectin and vitronectin. The interaction was mediated by integrins α5β3, αvβ1, and αvβ3. This study has demonstrated that an injured local environment presents sufficient adhesive signals to capture flow perfused LO-EPC in vitro and that LO-EPC have properties consistent with their potential role in vascular repair.
dc.rightsAll Rights Reserveden
dc.rights.urihttps://www.rioxx.net/licenses/all-rights-reserved/en
dc.titleDisrupted Endothelial Cell Layer and Exposed Extracellular Matrix Proteins Promote Capture of Late Outgrowth Endothelial Progenitor Cells
dc.typeArticle
dc.date.updated2017-07-11T12:47:24Z
dc.description.versionPeer Reviewed
dc.language.rfc3066en
dc.rights.holderCopyright © 2016 Jing Zhao et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.identifier.doi10.17863/CAM.13602
rioxxterms.versionofrecord10.1155/2016/1406304


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