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dc.contributor.authorTanpure, Arunen
dc.contributor.authorBalasubramanian, Shankaren
dc.date.accessioned2017-11-08T13:47:09Z
dc.date.available2017-11-08T13:47:09Z
dc.identifier.issn1439-4227
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/268176
dc.description.abstractThe synthesis of 2′-O-methyl-5-hydroxymethylcytidine (hm5Cm), 5-hydroxymethylcytidine (hm5C) and 5-formylcytidine (f5C) phosphoramidite monomers has been developed. Optimisation of mild post-synthetic deprotection conditions enabled the synthesis of RNA containing all four naturally occurring cytosine modifications (hm5Cm, hm5C, f5C plus 5-methylcytosine). Given the considerable interest in RNA modifications and epitranscriptomics, the availability of synthetic monomers and RNAs containing these modifications will be valuable for elucidating their biological function(s).
dc.description.sponsorshipWe acknowledge support from the University of Cambridge and Cancer Research UK program. The Balasubramanian laboratory is supported by core funding from Cancer Research UK (C14303/A17197). S.B. is a Senior Investigator of the Wellcome Trust (grant no. 099232/z /12/z).
dc.publisherWiley-Blackwell
dc.titleSynthesis and Multiple Incorporations of 2′-O-Methyl-5-hydroxymethylcytidine, 5-Hydroxymethylcytidine and 5-Formylcytidine Monomers into RNA Oligonucleotidesen
dc.typeArticle
prism.publicationNameChemBioChemen
dc.identifier.doi10.17863/CAM.14376
dcterms.dateAccepted2017-09-13en
rioxxterms.versionofrecord10.1002/cbic.201700492en
rioxxterms.versionAM*
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2017-09-13en
dc.contributor.orcidTanpure, Arun [0000-0002-5227-8376]
dc.contributor.orcidBalasubramanian, Shankar [0000-0002-0281-5815]
dc.identifier.eissn1439-7633
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idWellcome Trust (099232/Z/12/Z)
pubs.funder-project-idCancer Research UK (CB4330)
cam.issuedOnline2017-09-13en
rioxxterms.freetoread.startdate2018-09-13


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