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dc.contributor.authorProtasio, Anna Ven
dc.contributor.authorvan Dongen, Stijnen
dc.contributor.authorCollins, Julieen
dc.contributor.authorQuintais, Leonoren
dc.contributor.authorRibeiro, Diogo Men
dc.contributor.authorSessler, Florianen
dc.contributor.authorHunt, Martinen
dc.contributor.authorRinaldi, Gabrielen
dc.contributor.authorCollins, James Jen
dc.contributor.authorEnright, Antonen
dc.contributor.authorBerriman, Matthewen
dc.date.accessioned2018-01-22T18:18:51Z
dc.date.available2018-01-22T18:18:51Z
dc.date.issued2017-05-23en
dc.identifier.issn1935-2727
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/270976
dc.description.abstractSchistosomes are parasitic helminths that cause schistosomiasis, a disease affecting circa 200 million people, primarily in underprivileged regions of the world. Schistosoma mansoni is the most experimentally tractable schistosome species due to its ease of propagation in the laboratory and the high quality of its genome assembly and annotation. Although there is growing interest in microRNAs (miRNAs) in trematodes, little is known about the role these molecules play in the context of developmental processes. We use the completely unaware "miRNA-blind" bioinformatics tool Sylamer to analyse the 3’-UTRs of transcripts differentially expressed between the juvenile and adult stages. We show that the miR-277/4989 family target sequence is the only one significantly enriched in the transition from juvenile to adult worms. Further, we describe a novel miRNA, sma-miR-4989 showing that its proximal genomic location to sma-miR-277 suggests that they form a miRNA cluster, and we propose hairpin folds for both miRNAs compatible with the miRNA pathway. In addition, we found that expression of sma-miR-277/4989 miRNAs are up-regulated in adults while their predicted targets are characterised by significant down-regulation in paired adult worms but remain largely undisturbed in immature "virgin" females. Finally, we show that sma-miR-4989 is expressed in tegumental cells located proximal to the oesophagus gland and also distributed throughout the male worms’ body. Our results indicate that sma-miR-277/4989 might play a dominant role in post-transcriptional regulation during development of juvenile worms and suggest an important role in the sexual development of female schistosomes.
dc.format.mediumElectronic-eCollectionen
dc.languageengen
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectAnimalsen
dc.subjectMice, Inbred BALB Cen
dc.subjectSchistosoma mansonien
dc.subjectMicroRNAsen
dc.subjectComputational Biologyen
dc.subjectTranscription, Geneticen
dc.subjectGene Expression Regulationen
dc.subjectFemaleen
dc.subjectMaleen
dc.titleMiR-277/4989 regulate transcriptional landscape during juvenile to adult transition in the parasitic helminth Schistosoma mansoni.en
dc.typeArticle
prism.issueIdentifier5en
prism.publicationDate2017en
prism.publicationNamePLoS neglected tropical diseasesen
prism.startingPagee0005559
prism.volume11en
dc.identifier.doi10.17863/CAM.17929
dcterms.dateAccepted2017-04-05en
rioxxterms.versionofrecord10.1371/journal.pntd.0005559en
rioxxterms.versionVoR*
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2017-05-23en
dc.contributor.orcidProtasio, Anna V [0000-0003-1723-800X]
dc.contributor.orcidRibeiro, Diogo M [0000-0002-0482-7342]
dc.contributor.orcidRinaldi, Gabriel [0000-0002-7767-4922]
dc.contributor.orcidEnright, Anton [0000-0002-6090-3100]
dc.contributor.orcidBerriman, Matthew [0000-0002-9581-0377]
dc.identifier.eissn1935-2735
rioxxterms.typeJournal Article/Reviewen


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International