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dc.contributor.authorCavallero, Serenaen
dc.contributor.authorLombardo, Fabrizioen
dc.contributor.authorSu, Xiaopeien
dc.contributor.authorSalvemini, Marcoen
dc.contributor.authorCantacessi, Cinziaen
dc.contributor.authorD'Amelio, Stefanoen
dc.date.accessioned2018-02-05T17:25:44Z
dc.date.available2018-02-05T17:25:44Z
dc.date.issued2018-01-10en
dc.identifier.issn1756-3305
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/271696
dc.description.abstractBackground: Larval stages of the sibling species of parasitic nematodes Anisakis simplex sensu stricto (AS) and Anisakis pegreffii (AP) are responsible for a fish-borne zoonosis, known as anisakiasis, that humans acquire via the ingestion of raw or undercooked infected fish or fish-based products. These two species differ in geographical distribution, genetic background and peculiar traits involved in pathogenicity. However, thus far, little is known of key molecules potentially involved in host-parasite interactions. Here, high-throughput RNA-Seq and bioinformatics analyses of sequence data were applied to the characterization of the whole sets of transcripts expressed by infective larvae of AS and AP, as well as of their pharyngeal tissues, in a bid to identify transcripts potentially involved in tissue invasion and host-pathogen interplay. Results: Approximately ~34,000,000 single-end reads were generated from cDNA libraries for each species. Transcripts identified in AS and AP encoded 19,403 and 10,424 putative peptides, respectively, and were classified based on homology searches, protein motifs, Gene Ontology and biological pathway mapping. Differential gene expression analysis yielded 226 and 339 transcripts up-regulated in the pharyngeal regions of AS and AP, respectively, compared with their corresponding whole-larvae datasets. These included proteolytic enzymes, molecules encoding anaesthetics, inhibitors of primary hemostasis and virulence factors, anticoagulants and immunomodulatory peptides. Conclusions: This work provides the scientific community with a list of key transcripts expressed by AS and AP pharyngeal tissues and corresponding annotation information which represents a ready-to-use resource for future functional studies of biological pathways specifically involved in host-parasite interplay.
dc.format.mediumElectronicen
dc.languageengen
dc.publisherBioMed Central
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectPharynxen
dc.subjectAnimalsen
dc.subjectFishesen
dc.subjectAnisakisen
dc.subjectAnisakiasisen
dc.subjectFish Diseasesen
dc.subjectPeptide Hydrolasesen
dc.subjectDNA, Helminthen
dc.subjectSequence Analysis, RNAen
dc.subjectComputational Biologyen
dc.subjectVirulenceen
dc.subjectSpecies Specificityen
dc.subjectLarvaen
dc.subjectPolymorphism, Restriction Fragment Lengthen
dc.subjectHost-Parasite Interactionsen
dc.subjectTranscriptomeen
dc.titleTissue-specific transcriptomes of Anisakis simplex (sensu stricto) and Anisakis pegreffii reveal potential molecular mechanisms involved in pathogenicity.en
dc.typeArticle
prism.issueIdentifier1en
prism.publicationDate2018en
prism.publicationNameParasites & vectorsen
prism.startingPage31
prism.volume11en
dc.identifier.doi10.17863/CAM.18685
dcterms.dateAccepted2017-12-11en
rioxxterms.versionofrecord10.1186/s13071-017-2585-7en
rioxxterms.versionVoR*
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2018-01-10en
dc.contributor.orcidSalvemini, Marco [0000-0003-2297-9267]
dc.contributor.orcidCantacessi, Cinzia [0000-0001-6863-2950]
dc.identifier.eissn1756-3305
rioxxterms.typeJournal Article/Reviewen
cam.orpheus.successThu Jan 30 13:00:37 GMT 2020 - The item has an open VoR version.*
rioxxterms.freetoread.startdate2100-01-01


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International