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dc.contributor.authorIllingworth, Christopheren
dc.contributor.authorRoy, Sunandoen
dc.contributor.authorBeale, Mathew Aen
dc.contributor.authorTutill, Helenaen
dc.contributor.authorWilliams, Rachelen
dc.contributor.authorBreuer, Judithen
dc.date.accessioned2018-05-02T08:34:46Z
dc.date.available2018-05-02T08:34:46Z
dc.date.issued2017-07en
dc.identifier.issn2057-1577
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/275402
dc.description.abstractGenome sequence data are of great value in describing evolutionary processes in viral populations. However, in such studies, the extent to which data accurately describes the viral population is a matter of importance. Multiple factors may influence the accuracy of a dataset, including the quantity and nature of the sample collected, and the subsequent steps in viral processing. To investigate this phenomenon, we sequenced replica datasets spanning a range of viruses, and in which the point at which samples were split was different in each case, from a dataset in which independent samples were collected from a single patient to another in which all processing steps up to sequencing were applied to a single sample before splitting the sample and sequencing each replicate. We conclude that neither a high read depth or a high template number in a sample guarantee the precision of a dataset. Measures of consistency calculated from within a single biological sample may also be insufficient; distortion of the composition of a population by the experimental procedure or genuine within-host diversity between samples may each affect the results. Where it is possible, data from replicate samples should be collected to validate the consistency of short-read sequence data.
dc.format.mediumElectronic-eCollectionen
dc.languageengen
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.titleOn the effective depth of viral sequence data.en
dc.typeArticle
prism.issueIdentifier2en
prism.publicationDate2017en
prism.publicationNameVirus evolutionen
prism.startingPagevex030
prism.volume3en
dc.identifier.doi10.17863/CAM.22607
dcterms.dateAccepted2017-09-20en
rioxxterms.versionofrecord10.1093/ve/vex030en
rioxxterms.versionVoR*
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2017-07en
dc.contributor.orcidIllingworth, Christopher [0000-0002-0030-2784]
dc.contributor.orcidBeale, Mathew A [0000-0002-4740-3187]
dc.contributor.orcidBreuer, Judith [0000-0001-8246-0534]
dc.identifier.eissn2057-1577
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idWellcome Trust (101239/Z/13/Z)
cam.orpheus.successThu Jan 30 13:00:14 GMT 2020 - The item has an open VoR version.*
rioxxterms.freetoread.startdate2100-01-01


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International