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dc.contributor.authorde Freitas Nascimento, Janainaen
dc.contributor.authorKelly, Stevenen
dc.contributor.authorSunter, Jacken
dc.contributor.authorCarrington, Marken
dc.date.accessioned2018-05-21T09:49:53Z
dc.date.available2018-05-21T09:49:53Z
dc.date.issued2018-03-15en
dc.identifier.issn2050-084X
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/275987
dc.description.abstractSelective transcription of individual protein coding genes does not occur in trypanosomes and the cellular copy number of each mRNA must be determined post-transcriptionally. Here, we provide evidence that codon choice dictates the levels of constitutively expressed mRNAs. First, a novel codon usage metric, the gene expression codon adaptation index (geCAI), was developed that maximised the relationship between codon choice and the measured abundance for a transcriptome. Second, geCAI predictions of mRNA levels were tested using differently coded GFP transgenes and were successful over a 25-fold range, similar to the variation in endogenous mRNAs. Third, translation was necessary for the accelerated mRNA turnover resulting from codon choice. Thus, in trypanosomes, the information determining the levels of most mRNAs resides in the open reading frame and translation is required to access this information.
dc.format.mediumElectronicen
dc.languageengen
dc.publishereLife Sciences Publications Ltd
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectTrypanosomaen
dc.subjectRNA, Messengeren
dc.subjectCodonen
dc.subjectProtein Biosynthesisen
dc.subjectTranscription, Geneticen
dc.subjectOpen Reading Framesen
dc.subjectTranscriptomeen
dc.titleCodon choice directs constitutive mRNA levels in trypanosomes.en
dc.typeArticle
prism.publicationDate2018en
prism.publicationNameeLifeen
prism.volume7en
dc.identifier.doi10.17863/CAM.23265
dcterms.dateAccepted2018-02-27en
rioxxterms.versionofrecord10.7554/elife.32467en
rioxxterms.versionVoR*
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/en
rioxxterms.licenseref.startdate2018-03-15en
dc.contributor.orcidde Freitas Nascimento, Janaina [0000-0002-1757-3441]
dc.contributor.orcidKelly, Steven [0000-0001-8583-5362]
dc.contributor.orcidSunter, Jack [0000-0002-2836-9622]
dc.contributor.orcidCarrington, David [0000-0002-6435-7266]
dc.identifier.eissn2050-084X
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idWellcome Trust (085956/Z/08/Z)
cam.orpheus.successThu Jan 30 13:00:12 GMT 2020 - The item has an open VoR version.*
rioxxterms.freetoread.startdate2100-01-01


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International