Show simple item record

dc.contributor.authorChan, Kher Xing
dc.date.accessioned2018-06-11T09:14:06Z
dc.date.available2018-06-11T09:14:06Z
dc.date.issued2019-05-18
dc.date.submitted2018-01-04
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/276829
dc.description.abstractChlamydomonas reinhardtii possesses a single-cell-based CO2-concentrating mechanism (CCM). The CCM is an important element of algal photosynthesis, metabolism, growth and biomass production, which works by increasing the concentration of inorganic carbon (Ci) in the pyrenoid, a dense RuBisCO-packed structure within the chloroplast. This suppresses RuBisCO oxygenase activity and associated photorespiration. The enhanced efficiency of CO2 assimilation in the pyrenoid via CCM had been modelled theoretically as a requirement for successful CCM in higher plant systems. The ultimate aim of my research is to understand the biogenesis of the pyrenoid using a set of CCM mutants with pyrenoidal defects. Immunofluorescence methods and spot growth tests under different CO2 concentrations were performed on mutants with CCM defects generated by an insertional mutagenesis screen. Morphological and physiological characterisation of these mutants revealed differences in the pyrenoid morphology, the ability for RuBisCO to aggregate into the pyrenoid and the formation of thylakoidal tubule network associated with the pyrenoid. The thylakoid tubule network may be linked to the transport of inorganic carbon into the pyrenoid as part of the CCM. Further characterisation of one of the mutants gave rise to the hypothesis that the gene of interest, Cre11.g467712 (SAGA), is a multi-functional anchor protein related to the structural formation of the pyrenoid and may be another essential component of the pyrenoid.
dc.description.sponsorshipBBSRC/NSF Combining Algal and Plant Photosynthesis (CAPP), Cambridge-IDB International Scholarship, Cambridge Trust PhD Overrun Fund, Cambridge Philosophical Society Research Studentship, Lundgren Research Award, Houston Putnam Lowry Fund - Fitzwilliam College
dc.language.isoen
dc.rightsAll rights reserved
dc.rightsAll Rights Reserveden
dc.rights.urihttps://www.rioxx.net/licenses/all-rights-reserved/en
dc.subjectChlamydomonas reinhardtii
dc.subjectCarbon concentrating mechanism
dc.subjectGreen alga
dc.subjectpyrenoid
dc.subjectThylakoid tubule network
dc.subjectCO2
dc.subjectcarbon dioxide
dc.subjectsingle-cell CCM
dc.subjectCCM
dc.subjectRubisco
dc.subjectImmunofluorescence
dc.subjectElectron microscopy
dc.subjectImmunogold labelling
dc.subjectWestern blotting
dc.subjectProteomics
dc.subjectMicroscopy
dc.titleMorphological and Physiological Studies of the Carbon Concentrating Mechanism in Chlamydomonas reinhardtii
dc.typeThesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnameDoctor of Philosophy (PhD)
dc.publisher.institutionUniversity of Cambridge
dc.publisher.departmentPlant Sciences
dc.date.updated2018-06-08T16:00:59Z
dc.identifier.doi10.17863/CAM.24108
dc.contributor.orcidChan, Kher Xing [0000-0002-0236-6378]
dc.publisher.collegeFitzwilliam College
dc.type.qualificationtitlePhD in Plant Sciences
cam.supervisorGriffiths, Howard
cam.thesis.fundingtrue
rioxxterms.freetoread.startdate2019-06-11


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record