Show simple item record

dc.contributor.authorHensel, Kai O
dc.contributor.authorCantner, Franziska
dc.contributor.authorBangert, Felix
dc.contributor.authorWirth, Stefan
dc.contributor.authorPostberg, Jan
dc.date.accessioned2018-06-23T06:04:28Z
dc.date.available2018-06-23T06:04:28Z
dc.date.issued2018-06-22
dc.identifier.citationEpigenetics & Chromatin. 2018 Jun 22;11(1):34
dc.identifier.issn1756-8935
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/277418
dc.description.abstractBACKGROUND: In hepatocyte nuclei, hepatitis B virus (HBV) genomes occur episomally as covalently closed circular DNA (cccDNA). The HBV X protein (HBx) is required to initiate and maintain HBV replication. The functional nuclear localization of cccDNA and HBx remains unexplored. RESULTS: To identify virus-host genome interactions and the underlying nuclear landscape for the first time, we combined circular chromosome conformation capture (4C) with RNA-seq and ChIP-seq. Moreover, we studied HBx-binding to HBV episomes. In HBV-positive HepaRG hepatocytes, we observed preferential association of HBV episomes and HBx with actively transcribed nuclear domains on the host genome correlating in size with constrained topological units of chromatin. Interestingly, HBx alone occupied transcribed chromatin domains. Silencing of native HBx caused reduced episomal HBV stability. CONCLUSIONS: As part of the HBV episome, HBx might stabilize HBV episomal nuclear localization. Our observations may contribute to the understanding of long-term episomal stability and the facilitation of viral persistence. The exact mechanism by which HBx contributes to HBV nuclear persistence warrants further investigations.
dc.publisherSpringer Science and Business Media LLC
dc.titleEpisomal HBV persistence within transcribed host nuclear chromatin compartments involves HBx.
dc.typeJournal Article
dc.date.updated2018-06-23T06:04:24Z
dc.language.rfc3066en
dc.rights.holderThe Author(s)
prism.publicationNameEpigenetics Chromatin
dc.identifier.doi10.17863/CAM.24727
dcterms.dateAccepted2018-06-19
rioxxterms.versionofrecord10.1186/s13072-018-0204-2
dc.contributor.orcidHensel, Kai O [0000-0001-5826-6133]
dc.contributor.orcidPostberg, Jan [0000-0002-8562-3316]
dc.identifier.eissn1756-8935
cam.issuedOnline2018-06-22


Files in this item

Thumbnail
Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record