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dc.contributor.authorJung, Peter
dc.contributor.authorSommer, Christian
dc.contributor.authorBarriga, Francisco M
dc.contributor.authorBuczacki, Simon
dc.contributor.authorHernando-Momblona, Xavier
dc.contributor.authorSevillano, Marta
dc.contributor.authorDuran-Frigola, Miquel
dc.contributor.authorAloy, Patrick
dc.contributor.authorSelbach, Matthias
dc.contributor.authorWinton, Douglas
dc.contributor.authorBatlle, Eduard
dc.date.accessioned2018-09-05T12:43:43Z
dc.date.available2018-09-05T12:43:43Z
dc.date.issued2015-12-08
dc.identifier.issn2213-6711
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/279437
dc.description.abstractInsertion of reporter cassettes into the Lgr5 locus has enabled the characterization of mouse intestinal stem cells (ISCs). However, low cell surface abundance of LGR5 protein and lack of high-affinity anti-LGR5 antibodies represent a roadblock to efficiently isolate human colonic stem cells (hCoSCs). We set out to identify stem cell markers that would allow for purification of hCoSCs. In an unbiased approach, membrane-enriched protein fractions derived from in vitro human colonic organoids were analyzed by quantitative mass spectrometry. Protein tyrosine pseudokinase PTK7 specified a cell population within human colonic organoids characterized by highest self-renewal and re-seeding capacity. Antibodies recognizing the extracellular domain of PTK7 allowed us to isolate and expand hCoSCs directly from patient-derived mucosa samples. Human PTK7+ cells display features of canonical Lgr5+ ISCs and include a fraction of cells that undergo differentiation toward enteroendocrine lineage that resemble crypt label retaining cells (LRCs).
dc.format.mediumPrint-Electronic
dc.languageeng
dc.publisherElsevier BV
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectColon
dc.subjectCells, Cultured
dc.subjectStem Cells
dc.subjectHumans
dc.subjectReceptor Protein-Tyrosine Kinases
dc.subjectCell Adhesion Molecules
dc.subjectOrgan Culture Techniques
dc.subjectCell Separation
dc.subjectCell Proliferation
dc.subjectMass Spectrometry
dc.titleIsolation of Human Colon Stem Cells Using Surface Expression of PTK7.
dc.typeArticle
prism.endingPage987
prism.issueIdentifier6
prism.publicationDate2015
prism.publicationNameStem Cell Reports
prism.startingPage979
prism.volume5
dc.identifier.doi10.17863/CAM.26811
dcterms.dateAccepted2015-10-08
rioxxterms.versionofrecord10.1016/j.stemcr.2015.10.003
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2015-12
dc.contributor.orcidBuczacki, Simon [0000-0002-2975-416X]
dc.contributor.orcidWinton, Douglas [0000-0001-6067-7927]
dc.identifier.eissn2213-6711
rioxxterms.typeJournal Article/Review
pubs.funder-project-idCancer Research UK (C14303/A17197)


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Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)
Except where otherwise noted, this item's licence is described as Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0)