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dc.contributor.authorHernandez, Rafael E
dc.contributor.authorGalitan, Louie
dc.contributor.authorCameron, James
dc.contributor.authorGoodwin, Nicola
dc.contributor.authorRamakrishnan, Lalita
dc.date.accessioned2018-09-05T12:50:54Z
dc.date.available2018-09-05T12:50:54Z
dc.date.issued2018-10
dc.identifier.issn1545-8547
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/279634
dc.description.abstractThe use of early-stage zebrafish for biomedical research spans early organogenesis to free-swimming larva. A key benefit of this model organism is that repeated assessments spanning several days can be performed of individual larvae within a single experiment, often in conjunction with administered drugs. However, the initiation of feeding, typically at 5 days postfertilization (dpf), can make serial assessments challenging. Therefore, delayed feeding would increase the utility of the model. To ask whether feeding could be delayed without adversely affecting larval growth and development up to 39 dpf, we systematically raised zebrafish and introduced feeding at 5 dpf or delayed initial feeding up to 9 dpf. We assessed survival into the juvenile stage (39 dpf) and anterior-posterior length at this age as proxies for growth and development. Delaying feeding initiation up to 8 dpf did not decrease baseline survival of greater than 90%; survival decreased to 66% only when delayed to 9 dpf. Larval length was no different under any of these conditions. Our findings define 9 dpf as the critical age before which larval zebrafish must be fed when raising to 39 dpf. The option to delay feeding to 8 dpf will broaden experimental applications for the zebrafish larval model.
dc.format.mediumPrint-Electronic
dc.languageeng
dc.publisherMary Ann Liebert Inc
dc.rightsAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectAnimals
dc.subjectZebrafish
dc.subjectSurvival Rate
dc.subjectFeeding Behavior
dc.subjectFood Deprivation
dc.subjectLarva
dc.subjectFertilization
dc.titleDelay of Initial Feeding of Zebrafish Larvae Until 8 Days Postfertilization Has No Impact on Survival or Growth Through the Juvenile Stage.
dc.typeArticle
prism.endingPage518
prism.issueIdentifier5
prism.publicationDate2018
prism.publicationNameZebrafish
prism.startingPage515
prism.volume15
dc.identifier.doi10.17863/CAM.25905
dcterms.dateAccepted2018-07-01
rioxxterms.versionofrecord10.1089/zeb.2018.1579
rioxxterms.versionNA
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2018-10
dc.contributor.orcidRamakrishnan, Lalita [0000-0003-0692-5533]
dc.identifier.eissn1557-8542
rioxxterms.typeJournal Article/Review
pubs.funder-project-idWellcome Trust (103950/Z/14/Z)


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Attribution 4.0 International (CC BY 4.0)
Except where otherwise noted, this item's licence is described as Attribution 4.0 International (CC BY 4.0)