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dc.contributor.authorSanchez-Corrales, Yara Een
dc.contributor.authorBlanchard, Guyen
dc.contributor.authorRöper, Katjaen
dc.date.accessioned2018-09-08T06:32:37Z
dc.date.available2018-09-08T06:32:37Z
dc.date.issued2018-07-17en
dc.identifier.issn2050-084X
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/279855
dc.description.abstractThe budding of tubular organs from flat epithelial sheets is a vital morphogenetic process. Cell behaviours that drive such processes are only starting to be unraveled. Using live-imaging and novel morphometric methods we show that in addition to apical constriction, radially-oriented directional intercalation of cells plays a major contribution to early stages of invagination of the salivary gland tube in the Drosophila embryo. Extending analyses in 3D, we find that near the pit of invagination, isotropic apical constriction leads to strong cell-wedging. Further from the pit cells interleave circumferentially, suggesting apically-driven behaviours. Supporting this, junctional myosin is enriched in, and neighbour exchanges are biased towards the circumferential orientation. In a mutant failing pit specification, neither are biased due to an inactive pit. Thus, tube budding involves radially-patterned pools of apical myosin, medial as well as junctional, and radially-patterned 3D-cell behaviours, with a close mechanical interplay between invagination and intercalation.
dc.format.mediumElectronicen
dc.languageengen
dc.publishereLife Sciences Publications Ltd
dc.rightsAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectSalivary Glandsen
dc.subjectEpitheliumen
dc.subjectEpithelial Cellsen
dc.subjectEmbryo, Nonmammalianen
dc.subjectAnimalsen
dc.subjectDrosophila melanogasteren
dc.subjectMyosin Type IIen
dc.subjectImaging, Three-Dimensionalen
dc.subjectCell Polarityen
dc.subjectCell Shapeen
dc.subjectMorphogenesisen
dc.subjectMutationen
dc.subjectFluorescenceen
dc.subjectTime-Lapse Imagingen
dc.titleRadially patterned cell behaviours during tube budding from an epithelium.en
dc.typeArticle
prism.publicationDate2018en
prism.publicationNameeLifeen
prism.volume7en
dc.identifier.doi10.17863/CAM.27223
dcterms.dateAccepted2018-07-16en
rioxxterms.versionofrecord10.7554/eLife.35717en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2018-07-17en
dc.contributor.orcidSanchez-Corrales, Yara E [0000-0003-1438-1994]
dc.contributor.orcidBlanchard, Guy [0000-0002-3689-0522]
dc.contributor.orcidRöper, Katja [0000-0002-3361-766X]
dc.identifier.eissn2050-084X
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idBBSRC (BB/J010278/1)
pubs.funder-project-idWellcome Trust (099234/Z/12/Z)
pubs.funder-project-idIsaac Newton Trust (Minute 1523(k))
pubs.funder-project-idWellcome Trust (100329/Z/12/Z)


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Attribution 4.0 International (CC BY 4.0)
Except where otherwise noted, this item's licence is described as Attribution 4.0 International (CC BY 4.0)