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dc.contributor.authorTrigg, RM
dc.contributor.authorMartinson, LJ
dc.contributor.authorParpart-Li, S
dc.contributor.authorShaw, JA
dc.date.accessioned2018-09-20T12:08:10Z
dc.date.available2018-09-20T12:08:10Z
dc.date.issued2018-07
dc.identifier.issn2405-8440
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/280580
dc.description.abstractBACKGROUND: Circulating-free DNA (cfDNA) is under investigation as a liquid biopsy of cancer for early detection, monitoring disease progression and therapeutic response. This systematic review of the primary cfDNA literature aims to identify and evaluate factors that influence recovery of cfDNA, and to outline evidence-based recommendations for standardization of methods. METHODS: A search of the Ovid and Cochrane databases was undertaken in May 2018 to obtain relevant literature on cfDNA isolation and quantification. Retrieved titles and abstracts were reviewed by two authors. The factors evaluated include choice of specimen type (plasma or serum); time-to-processing of whole blood; blood specimen tube; centrifugation protocol (speed, time, temperature and number of spins); and methods of cfDNA isolation and quantification. FINDINGS: Of 4,172 articles identified through the database search, 52 proceeded to full-text review and 37 met the criteria for inclusion. A quantitative analysis was not possible, due to significant heterogeneity in methodological approaches between studies. Therefore, included data was tabulated and a textual qualitative synthesis approach was taken. INTERPRETATION: This is the first systematic review of methodological factors that influence recovery and quantification of cfDNA, enabling recommendations to be made that will support standardization of methodological approaches towards development of blood-based cancer tests.
dc.format.mediumElectronic-eCollection
dc.languageeng
dc.publisherElsevier BV
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.titleFactors that influence quality and yield of circulating-free DNA: A systematic review of the methodology literature.
dc.typeArticle
prism.issueIdentifier7
prism.publicationDate2018
prism.publicationNameHeliyon
prism.startingPagee00699
prism.volume4
dc.identifier.doi10.17863/CAM.27948
dcterms.dateAccepted2018-07-13
rioxxterms.versionofrecord10.1016/j.heliyon.2018.e00699
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2018-07-25
dc.contributor.orcidTrigg, Ricky [0000-0001-9329-9344]
dc.identifier.eissn2405-8440
rioxxterms.typeJournal Article/Review
cam.issuedOnline2018-07-25


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Attribution-NonCommercial-NoDerivatives 4.0 International
Except where otherwise noted, this item's licence is described as Attribution-NonCommercial-NoDerivatives 4.0 International