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dc.contributor.authorThackray, AM
dc.contributor.authorAndréoletti, O
dc.contributor.authorBujdoso, R
dc.date.accessioned2018-09-25T08:33:52Z
dc.date.available2018-09-25T08:33:52Z
dc.date.issued2018
dc.identifier.issn2046-1402
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/280691
dc.description.abstractPrion diseases are fatal neurodegenerative conditions of humans and vertebrate species. The transmissible prion agent is a novel infectious particle composed principally of PrP Sc, an abnormal isomer of the normal host protein PrP C. The only reliable method to detect mammalian prion infectivity is by bioassay, invariably in a vertebrate host. The current prion bioassays typically involve intracerebral or peripheral inoculation of test material into the experimental host and subsequent euthanasia when clinical signs of terminal prion disease become evident. It may be months or years before the onset of clinical disease becomes evident and a pre-determined clinical end-point is reached. Consequently, bioassay of prion infectivity in vertebrate species is cumbersome, time consuming, expensive, and increasingly open to ethical debate because these animals are subjected to terminal neurodegenerative disease. Prions are a significant risk to public health through the potential for zoonotic transmission of animal prion diseases. Attention has focussed on the measurement of prion infectivity in different tissues and blood from prion-infected individuals in order to determine the distribution of infectious prions in diseased hosts. New animal models are required in order to replace or reduce, where possible, the dependency on the use of vertebrate species, including the 'gold standard' mouse prion bioassay, to assess prion infectivity levels. Here we highlight the development of a Drosophila-based prion bioassay, a highly sensitive and rapid invertebrate animal system that can efficiently detect mammalian prions. This novel invertebrate model system will be of considerable interest to biologists who perform prion bioassays as it will promote reduction and replacement in the number of sentient animals currently used for this purpose. This article is a composite of previous methods that provides an overview of the methodology of the model and discusses the experimental data to promote its viability for use instead of more sentient hosts.
dc.languageeng
dc.publisherF1000 Research Ltd
dc.rightsAttribution 4.0 International
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subjectprion
dc.subjectinfectivity
dc.subjectbioassay
dc.subjectinvertebrate
dc.subjectDrosophila
dc.titleThe use of PrP transgenic Drosophila to replace and reduce vertebrate hosts in the bioassay of mammalian prion infectivity [version 1; referees: 2 approved]
dc.typeArticle
prism.publicationNameF1000Research
prism.volume7
dc.identifier.doi10.17863/CAM.28056
dcterms.dateAccepted2018-05-15
rioxxterms.versionofrecord10.12688/f1000research.14753.1
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
rioxxterms.licenseref.startdate2018-05-15
dc.contributor.orcidThackray, Alana [0000-0002-2752-1127]
dc.contributor.orcidBujdoso, Raymond [0000-0002-5068-3247]
dc.identifier.eissn1759-796X
rioxxterms.typeJournal Article/Review
pubs.funder-project-idNational Centre for the Replacement Refinement and Reduction of Animals in Research (NC/R00093X/1)
cam.issuedOnline2018-05-15
cam.orpheus.successThu Jan 30 10:54:21 GMT 2020 - The item has an open VoR version.
rioxxterms.freetoread.startdate2100-01-01


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International