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dc.contributor.authorLanders, Courtney Amaryllis
dc.date.accessioned2018-10-23T11:39:50Z
dc.date.available2018-10-23T11:39:50Z
dc.date.issued2019-05-18
dc.date.submitted2017-11-28
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/284358
dc.description.abstractPuerperal Psychosis is the most severe form of postnatal psychiatric illness, and is a psychiatric emergency. Human-based research to find a means of genetically predicting a woman’s risk of puerperal psychosis has so far failed to reliably or reproducibly identify candidate genes or pathways, due to problems common within the field of psychiatric genetics, leading to the proposal of using an animal model in the form of Porcine Maternal Infanticide. In this project, the author has aimed to understand the pathophysiology of PMI using NGS technologies in order to 1) extend the validity of PMI as a model for PP; 2) determine future steps for development of the PMI model; and 3) generate insights into the management of PMI (and by extension PP) via prediction and detection of a puerperal trigger. These aims have been pursued via two experiments. In the first, the author has created RNA-Seq libraries from archival RNA, and then performed differential gene expression analysis. The results indicated that RNA-Seq technologies can be used with archival RNA samples, but using such samples introduces the risk of degradation-based bias. The substantial influence of outliers, confounding factors and sample size on the results prevented reliable identification of candidate genes„ but provide concrete guidelines development of the Porcine Maternal Infanticide model. In the second, the author has created MBD-Seq libraries from archival tissue, and then performed differential methylation analysis. The results indicated that it is possible to use MBD-Seq technologies with Sus scrofa brain tissue. Once again, the effect of confounding factors and sample size on the results prevented reliable identification of candidate genes, but provide guidance for development of the Porcine Maternal Infanticide model.
dc.language.isoen
dc.rightsAll rights reserved
dc.rightsAll Rights Reserveden
dc.rights.urihttps://www.rioxx.net/licenses/all-rights-reserved/en
dc.subjectpuerperal psychosis
dc.subjectpost-natal psychosis
dc.subjectpostnatal psychosis
dc.subjectbipolar disorder
dc.subjectmaternal savaging
dc.subjectporcine maternal infanticide
dc.titleUsing a Porcine Maternal Infanticide Model to Investigate the Inheritance of Human Puerperal Psychosis
dc.typeThesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnameDoctor of Philosophy (PhD)
dc.publisher.institutionUniversity of Cambridge
dc.publisher.departmentPathology
dc.date.updated2018-10-23T02:10:51Z
dc.identifier.doi10.17863/CAM.31731
dc.publisher.collegePembroke College
dc.type.qualificationtitlePhD in Pathology
cam.supervisorSargent, Carole Anne
cam.supervisorAffara, Nabeel
cam.thesis.fundingfalse


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