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dc.contributor.authorSurmacki, Jakub Men
dc.contributor.authorWoodhams, Benjaminen
dc.contributor.authorHaslehurst, Alexandriaen
dc.contributor.authorPonder, Bruceen
dc.contributor.authorBohndiek, Sarahen
dc.date.accessioned2018-11-05T10:24:47Z
dc.date.available2018-11-05T10:24:47Z
dc.date.issued2018-08-22en
dc.identifier.issn2045-2322
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/284568
dc.description.abstractLive cell Raman micro-spectroscopy is emerging as a promising bioanalytical technique for label-free discrimination of a range of different cell types (e.g. cancer cells and fibroblasts) and behaviors (e.g. apoptosis). The aim of this study was to determine whether confocal Raman micro-spectroscopy shows sufficient sensitivity and specificity for identification of primary human bronchial epithelial cells (HBECs) to be used for live cell biological studies in vitro. We first compared cell preparation substrates and media, considering their influence on lung cell proliferation and Raman spectra, as well as methods for data acquisition, using different wavelengths (488nm, 785nm) and scan protocols (line, area). Evaluating these parameters using human lung cancer (A549) and fibroblast (MRC5) cell lines confirmed that line-scan data acquisition at 785 nm using complete cell media on a quartz substrate gave optimal performance. We then applied our protocol to acquisition of data from primary human bronchial epithelial cells (HBEC) derived from three independent sources, revealing an average sensitivity for different cell types of 96.3% and specificity of 95.2%. These results suggest that Raman micro-spectroscopy is suitable for differentiating primary HBEC cell cultures, which in future could be used for identifying different lung cell types within co-cultures and studying the process of early carcinogenesis in lung cell culture.
dc.format.mediumElectronicen
dc.languageengen
dc.publisherNature Publishing Group
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectLungen
dc.subjectBronchien
dc.subjectCell Lineen
dc.subjectFibroblastsen
dc.subjectEpithelial Cellsen
dc.subjectHumansen
dc.subjectLung Neoplasmsen
dc.subjectSpectrum Analysis, Ramanen
dc.subjectCell Culture Techniquesen
dc.subjectCoculture Techniquesen
dc.subjectSensitivity and Specificityen
dc.subjectCell Proliferationen
dc.subjectCarcinogenesisen
dc.subjectA549 Cellsen
dc.titleRaman micro-spectroscopy for accurate identification of primary human bronchial epithelial cells.en
dc.typeArticle
prism.issueIdentifier1en
prism.publicationDate2018en
prism.publicationNameScientific reportsen
prism.startingPage12604
prism.volume8en
dc.identifier.doi10.17863/CAM.31944
dcterms.dateAccepted2018-07-20en
rioxxterms.versionofrecord10.1038/s41598-018-30407-8en
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2018-08-22en
dc.contributor.orcidSurmacki, Jakub M [0000-0001-6654-0286]
dc.contributor.orcidWoodhams, Benjamin [0000-0003-2801-5733]
dc.contributor.orcidBohndiek, Sarah [0000-0003-0371-8635]
dc.identifier.eissn2045-2322
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idEuropean Commission (630729)
pubs.funder-project-idCancer Research UK (16267)
pubs.funder-project-idCancer Research UK (C14303/A17197)
pubs.funder-project-idCancer Research UK (C14303_do not transfer)
pubs.funder-project-idEPSRC (EP/N014588/1)


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International