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dc.contributor.authorOtchere, Isaac Darko
dc.contributor.authorCoscollá, Mireia
dc.contributor.authorSánchez-Busó, Leonor
dc.contributor.authorAsante-Poku, Adwoa
dc.contributor.authorBrites, Daniela
dc.contributor.authorLoiseau, Chloe
dc.contributor.authorMeehan, Conor
dc.contributor.authorOsei-Wusu, Stephen
dc.contributor.authorForson, Audrey
dc.contributor.authorLaryea, Clement
dc.contributor.authorYahayah, Abdallah Iddrisu
dc.contributor.authorBaddoo, Akosua
dc.contributor.authorAnsa, Gloria Akosua
dc.contributor.authorAboagye, Samuel Yaw
dc.contributor.authorAsare, Prince
dc.contributor.authorBorrell, Sonia
dc.contributor.authorGehre, Florian
dc.contributor.authorBeckert, Patrick
dc.contributor.authorKohl, Thomas A
dc.contributor.authorN'dira, Sanoussi
dc.contributor.authorBeisel, Christian
dc.contributor.authorAntonio, Martin
dc.contributor.authorNiemann, Stefan
dc.contributor.authorde Jong, Bouke C
dc.contributor.authorParkhill, Julian
dc.contributor.authorHarris, Simon R
dc.contributor.authorGagneux, Sebastien
dc.contributor.authorYeboah-Manu, Dorothy
dc.date.accessioned2018-11-23T00:31:53Z
dc.date.available2018-11-23T00:31:53Z
dc.date.issued2018-07-26
dc.identifier.issn2045-2322
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/285797
dc.description.abstractMycobacterium africanum (Maf) causes a substantial proportion of human tuberculosis in some countries of West Africa, but little is known on this pathogen. We compared the genomes of 253 Maf clinical isolates from Ghana, including N = 175 Lineage 5 (L5) and N = 78 Lineage 6 (L6). We found that the genomic diversity of L6 was higher than in L5 despite the smaller sample size. Regulatory proteins appeared to evolve neutrally in L5 but under purifying selection in L6. Even though over 90% of the human T cell epitopes were conserved in both lineages, L6 showed a higher ratio of non-synonymous to synonymous single nucleotide variation in these epitopes overall compared to L5. Of the 10% human T cell epitopes that were variable, most carried mutations that were lineage-specific. Our findings indicate that Maf L5 and L6 differ in some of their population genomic characteristics, possibly reflecting different selection pressures linked to distinct ecological niches.
dc.format.mediumElectronic
dc.languageeng
dc.publisherSpringer Science and Business Media LLC
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectHumans
dc.subjectMycobacterium
dc.subjectTuberculosis
dc.subjectGenomics
dc.subjectGenotype
dc.subjectGenome, Bacterial
dc.subjectGhana
dc.subjectGenetic Variation
dc.titleComparative genomics of Mycobacterium africanum Lineage 5 and Lineage 6 from Ghana suggests distinct ecological niches.
dc.typeArticle
prism.issueIdentifier1
prism.publicationDate2018
prism.publicationNameSci Rep
prism.startingPage11269
prism.volume8
dc.identifier.doi10.17863/CAM.33141
dcterms.dateAccepted2018-07-16
rioxxterms.versionofrecord10.1038/s41598-018-29620-2
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2018-07-26
dc.contributor.orcidOtchere, Isaac Darko [0000-0001-8982-9488]
dc.contributor.orcidSánchez-Busó, Leonor [0000-0002-4162-0228]
dc.contributor.orcidAsante-Poku, Adwoa [0000-0001-8929-193X]
dc.contributor.orcidParkhill, Julian [0000-0002-7069-5958]
dc.contributor.orcidHarris, Simon R [0000-0003-1512-6194]
dc.identifier.eissn2045-2322
rioxxterms.typeJournal Article/Review
cam.issuedOnline2018-07-26


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International