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dc.contributor.authorTeasdale, Matthew
dc.contributor.authorvan Doorn, NL
dc.contributor.authorFiddyment, Sarah
dc.contributor.authorWebb, CC
dc.contributor.authorO'Connor, T
dc.contributor.authorHofreiter, M
dc.contributor.authorCollins, Matthew
dc.contributor.authorBradley, DG
dc.date.accessioned2018-11-23T00:33:18Z
dc.date.available2018-11-23T00:33:18Z
dc.date.issued2015-01-19
dc.identifier.issn0962-8436
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/285853
dc.description.abstractParchment represents an invaluable cultural reservoir. Retrieving an additional layer of information from these abundant, dated livestock-skins via the use of ancient DNA (aDNA) sequencing has been mooted by a number of researchers. However, prior PCR-based work has indicated that this may be challenged by cross-individual and cross-species contamination, perhaps from the bulk parchment preparation process. Here we apply next generation sequencing to two parchments of seventeenth and eighteenth century northern English provenance. Following alignment to the published sheep, goat, cow and human genomes, it is clear that the only genome displaying substantial unique homology is sheep and this species identification is confirmed by collagen peptide mass spectrometry. Only 4% of sequence reads align preferentially to a different species indicating low contamination across species. Moreover, mitochondrial DNA sequences suggest an upper bound of contamination at 5%. Over 45% of reads aligned to the sheep genome, and even this limited sequencing exercise yield 9 and 7% of each sampled sheep genome post filtering, allowing the mapping of genetic affinity to modern British sheep breeds. We conclude that parchment represents an excellent substrate for genomic analyses of historical livestock.
dc.format.mediumPrint
dc.languageeng
dc.publisherThe Royal Society
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectSkin
dc.subjectAnimals
dc.subjectSheep
dc.subjectDNA
dc.subjectSequence Alignment
dc.subjectSpecies Specificity
dc.subjectBase Sequence
dc.subjectFossils
dc.subjectHistory, 17th Century
dc.subjectHistory, 18th Century
dc.subjectMolecular Sequence Data
dc.subjectEngland
dc.subjectMass Spectrometry
dc.subjectLivestock
dc.subjectHigh-Throughput Nucleotide Sequencing
dc.titlePaging through history: parchment as a reservoir of ancient DNA for next generation sequencing.
dc.typeArticle
prism.issueIdentifier1660
prism.publicationDate2015
prism.publicationNamePhilos Trans R Soc Lond B Biol Sci
prism.startingPage20130379
prism.volume370
dc.identifier.doi10.17863/CAM.33197
rioxxterms.versionofrecord10.1098/rstb.2013.0379
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2015-01
dc.contributor.orcidTeasdale, Matthew [0000-0002-7376-9975]
dc.contributor.orcidFiddyment, Sarah [0000-0002-8991-2318]
dc.contributor.orcidCollins, Matthew [0000-0003-4226-5501]
dc.identifier.eissn1471-2970
rioxxterms.typeJournal Article/Review
cam.issuedOnline2015-01-19


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International