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dc.contributor.authorHouldcroft, Charlotte J
dc.contributor.authorPetrova, Velislava
dc.contributor.authorLiu, Jimmy Z
dc.contributor.authorFrampton, Dan
dc.contributor.authorAnderson, Carl A
dc.contributor.authorGall, Astrid
dc.contributor.authorKellam, Paul
dc.date.accessioned2018-12-01T00:30:33Z
dc.date.available2018-12-01T00:30:33Z
dc.date.issued2014
dc.identifier.issn1932-6203
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/286193
dc.description.abstractLymphoblastoid cell lines (LCLs) are commonly used in molecular genetics, supplying DNA for the HapMap and 1000 Genomes Projects, used to test chemotherapeutic agents, and informing the basis of a number of population genetics studies of gene expression. The process of transforming human B cells into LCLs requires the presence of Epstein-Barr virus (EBV), a double-stranded DNA virus which through B-cell immortalisation maintains an episomal virus genome in every cell of an LCL at variable copy numbers. Previous studies have reported that EBV alters host-gene expression and EBV copy number may be under host genetic control. We performed a genome-wide association study of EBV genome copy number in LCLs and found the phenotype to be highly heritable, although no individual SNPs achieved a significant association with EBV copy number. The expression of two host genes (CXCL16 and AGL) was positively correlated and expression of ADARB2 was negatively correlated with EBV copy number in a genotype-independent manner. This study shows an association between EBV copy number and the gene expression profile of LCLs, and suggests that EBV copy number should be considered as a covariate in future studies of host gene expression in LCLs.
dc.format.mediumElectronic-eCollection
dc.languageeng
dc.publisherPublic Library of Science (PLoS)
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectB-Lymphocytes
dc.subjectCell Line, Transformed
dc.subjectHumans
dc.subjectHerpesvirus 4, Human
dc.subjectEpstein-Barr Virus Infections
dc.subjectGene Expression Regulation
dc.subjectGene Dosage
dc.subjectPolymorphism, Single Nucleotide
dc.subjectQuantitative Trait Loci
dc.subjectGenetic Variation
dc.subjectGenome-Wide Association Study
dc.subjectTranscriptome
dc.titleHost genetic variants and gene expression patterns associated with Epstein-Barr virus copy number in lymphoblastoid cell lines.
dc.typeArticle
prism.issueIdentifier10
prism.publicationDate2014
prism.publicationNamePLoS One
prism.startingPagee108384
prism.volume9
dc.identifier.doi10.17863/CAM.33505
dcterms.dateAccepted2014-08-20
rioxxterms.versionofrecord10.1371/journal.pone.0108384
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2014-01
dc.contributor.orcidHouldcroft, Charlotte [0000-0002-1833-5285]
dc.identifier.eissn1932-6203
rioxxterms.typeJournal Article/Review
pubs.funder-project-idEuropean Research Council (261213)
cam.issuedOnline2014-10-07


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International