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dc.contributor.authorMartins, Bruno MC
dc.contributor.authorTooke, Amy K
dc.contributor.authorThomas, Philipp
dc.contributor.authorLocke, James
dc.date.accessioned2018-12-08T00:30:50Z
dc.date.available2018-12-08T00:30:50Z
dc.date.issued2018-11-27
dc.identifier.issn0027-8424
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/286522
dc.description.abstractHow cells maintain their size has been extensively studied under constant conditions. In the wild, however, cells rarely experience constant environments. Here, we examine how the 24-h circadian clock and environmental cycles modulate cell size control and division timings in the cyanobacterium Synechococcus elongatus using single-cell time-lapse microscopy. Under constant light, wild-type cells follow an apparent sizer-like principle. Closer inspection reveals that the clock generates two subpopulations, with cells born in the subjective day following different division rules from cells born in subjective night. A stochastic model explains how this behavior emerges from the interaction of cell size control with the clock. We demonstrate that the clock continuously modulates the probability of cell division throughout day and night, rather than solely applying an on-off gate to division, as previously proposed. Iterating between modeling and experiments, we go on to identify an effective coupling of the division rate to time of day through the combined effects of the environment and the clock on cell division. Under naturally graded light-dark cycles, this coupling narrows the time window of cell divisions and shifts divisions away from when light levels are low and cell growth is reduced. Our analysis allows us to disentangle, and predict the effects of, the complex interactions between the environment, clock, and cell size control.
dc.description.sponsorshipERC Gatsby
dc.format.mediumPrint-Electronic
dc.languageeng
dc.publisherProceedings of the National Academy of Sciences
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectSynechococcus
dc.subjectEnvironment
dc.subjectEcosystem
dc.subjectCell Division
dc.subjectCell Size
dc.subjectLight
dc.subjectModels, Biological
dc.subjectCircadian Clocks
dc.titleCell size control driven by the circadian clock and environment in cyanobacteria.
dc.typeArticle
prism.endingPageE11424
prism.issueIdentifier48
prism.publicationDate2018
prism.publicationNameProc Natl Acad Sci U S A
prism.startingPageE11415
prism.volume115
dc.identifier.doi10.17863/CAM.33832
rioxxterms.versionofrecord10.1073/pnas.1811309115
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2018-11-08
dc.contributor.orcidMartins, Bruno MC [0000-0002-9730-7425]
dc.contributor.orcidTooke, Amy K [0000-0002-8719-5263]
dc.contributor.orcidThomas, Philipp [0000-0003-4919-8452]
dc.contributor.orcidLocke, James [0000-0003-0670-1943]
dc.identifier.eissn1091-6490
rioxxterms.typeJournal Article/Review
pubs.funder-project-idEuropean Research Council (338060)
pubs.funder-project-idBiotechnology and Biological Sciences Research Council (BB/L014130/1)
pubs.funder-project-idGatsby Charitable Foundation (unknown)
pubs.funder-project-idHuman Frontier Science Program (HFSP) (CDA-/2012)
cam.issuedOnline2018-11-08
datacite.issupplementedby.urlhttps://doi.org/10.17863/CAM.31834


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International