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dc.contributor.authorWeng, Chenchunen
dc.contributor.authorKosalka, Joannaen
dc.contributor.authorBerkyurek, Ahmeten
dc.contributor.authorStempor, Przemyslawen
dc.contributor.authorFeng, Xuezhuen
dc.contributor.authorMao, Huien
dc.contributor.authorZeng, Chenmingen
dc.contributor.authorLi, Wen-Junen
dc.contributor.authorYan, Yong-Hongen
dc.contributor.authorDong, Meng-Qiuen
dc.contributor.authorMorero, Natalia Rosalíaen
dc.contributor.authorZuliani, Ceciliaen
dc.contributor.authorBarabas, Orsolyaen
dc.contributor.authorAhringer, Julieen
dc.contributor.authorGuang, Shouhongen
dc.contributor.authorMiska, Ericen
dc.date.accessioned2019-01-09T00:31:57Z
dc.date.available2019-01-09T00:31:57Z
dc.date.issued2019-01en
dc.identifier.issn0890-9369
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/287701
dc.description.abstractPiwi-interacting RNAs (piRNAs) engage Piwi proteins to suppress transposons and nonself nucleic acids and maintain genome integrity and are essential for fertility in a variety of organisms. In Caenorhabditis elegans, most piRNA precursors are transcribed from two genomic clusters that contain thousands of individual piRNA transcription units. While a few genes have been shown to be required for piRNA biogenesis, the mechanism of piRNA transcription remains elusive. Here we used functional proteomics approaches to identify an upstream sequence transcription complex (USTC) that is essential for piRNA biogenesis. The USTC contains piRNA silencing-defective 1 (PRDE-1), SNPC-4, twenty-one-U fouled-up 4 (TOFU-4), and TOFU-5. The USTC forms unique piRNA foci in germline nuclei and coats the piRNA cluster genomic loci. USTC factors associate with the Ruby motif just upstream of type I piRNA genes. USTC factors are also mutually dependent for binding to the piRNA clusters and forming the piRNA foci. Interestingly, USTC components bind differentially to piRNAs in the clusters and other noncoding RNA genes. These results reveal the USTC as a striking example of the repurposing of a general transcription factor complex to aid in genome defense against transposons.
dc.format.mediumPrint-Electronicen
dc.languageengen
dc.publisherCold Spring Harbor Laboratory Press
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectAnimalsen
dc.subjectCaenorhabditis elegansen
dc.subjectCaenorhabditis elegans Proteinsen
dc.subjectRNA, Small Interferingen
dc.subjectProteomicsen
dc.subjectGene Expression Regulationen
dc.subjectAmino Acid Motifsen
dc.subjectProtein Bindingen
dc.subjectGenome, Helminthen
dc.titleThe USTC co-opts an ancient machinery to drive piRNA transcription in C. elegans.en
dc.typeArticle
prism.endingPage102
prism.issueIdentifier1-2en
prism.publicationDate2019en
prism.publicationNameGenes & developmenten
prism.startingPage90
prism.volume33en
dc.identifier.doi10.17863/CAM.35015
dcterms.dateAccepted2018-10-19en
rioxxterms.versionofrecord10.1101/gad.319293.118en
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2019-01en
dc.contributor.orcidStempor, Przemyslaw [0000-0002-9464-7475]
dc.contributor.orcidZuliani, Cecilia [0000-0001-6026-7207]
dc.contributor.orcidBarabas, Orsolya [0000-0002-2873-5872]
dc.contributor.orcidAhringer, Julie [0000-0002-7074-4051]
dc.contributor.orcidMiska, Eric [0000-0002-4450-576X]
dc.identifier.eissn1549-5477
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idEuropean Commission Horizon 2020 (H2020) Marie Sk?odowska-Curie actions (747666)
pubs.funder-project-idCancer Research UK (18583)
pubs.funder-project-idCancer Research UK (A14492)
pubs.funder-project-idWELLCOME TRUST (104640/Z/14/Z)
pubs.funder-project-idWellcome Trust (092096/Z/10/Z)


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International