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dc.contributor.authorGladrow, J
dc.contributor.authorRibezzi-Crivellari, M
dc.contributor.authorRitort, F
dc.contributor.authorKeyser, UF
dc.date.accessioned2019-01-18T00:30:31Z
dc.date.available2019-01-18T00:30:31Z
dc.date.issued2019-01-04
dc.identifier.issn2041-1723
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/288146
dc.description.abstractWhile thermal rates of state transitions in classical systems have been studied for almost a century, associated transition-path times have only recently received attention. Uphill and downhill transition paths between states at different free energies should be statistically indistinguishable. Here, we systematically investigate transition-path-time symmetry and report evidence of its breakdown on the molecular- and meso-scale out of equilibrium. In automated Brownian dynamics experiments, we establish first-passage-time symmetries of colloids driven by femtoNewton forces in holographically-created optical landscapes confined within microchannels. Conversely, we show that transitions which couple in a path-dependent manner to fluctuating forces exhibit asymmetry. We reproduce this asymmetry in folding transitions of DNA-hairpins driven out of equilibrium and suggest a topological mechanism of symmetry breakdown. Our results are relevant to measurements that capture a single coordinate in a multidimensional free energy landscape, as encountered in electrophysiology and single-molecule fluorescence experiments.
dc.description.sponsorship(J.G.) European Training Network (ETN) Grant No. 674979-NANOTRANS (J.G.) Winton Programme for the Physics of Sustainability (M.R.C.) European Unions Horizon 2020 Marie Skłodowska-Curie grant agreement No. 749944 (U.F.K.) ERC Consolidator Grant (DesignerPores 647144) (F.R.) Spanish Research Council [FIS2016-80458-P] (F.R.) Catalan Government [Icrea Academia prize 2013] (F.R.) EU [Proseqo, FETOPEN, Proposal 687089]
dc.format.mediumElectronic
dc.languageeng
dc.publisherSpringer Science and Business Media LLC
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectDNA
dc.subjectProtein Folding
dc.subjectKinetics
dc.subjectThermodynamics
dc.subjectModels, Chemical
dc.subjectModels, Molecular
dc.subjectInverted Repeat Sequences
dc.subjectMolecular Dynamics Simulation
dc.titleExperimental evidence of symmetry breaking of transition-path times.
dc.typeArticle
prism.issueIdentifier1
prism.publicationDate2019
prism.publicationNameNat Commun
prism.startingPage55
prism.volume10
dc.identifier.doi10.17863/CAM.35462
dcterms.dateAccepted2018-11-11
rioxxterms.versionofrecord10.1038/s41467-018-07873-9
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2019-01-04
dc.contributor.orcidKeyser, UF [0000-0003-3188-5414]
dc.identifier.eissn2041-1723
rioxxterms.typeJournal Article/Review
pubs.funder-project-idEuropean Commission Horizon 2020 (H2020) Marie Sk?odowska-Curie actions (674979)
pubs.funder-project-idEuropean Research Council (647144)
cam.issuedOnline2019-01-04
rioxxterms.freetoread.startdate2100-01-01


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International