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dc.contributor.authorOdom, Duncanen
dc.contributor.authorLukk, Margusen
dc.contributor.authorLS Jacquin, Adrienen
dc.date.accessioned2019-02-02T00:30:31Z
dc.date.available2019-02-02T00:30:31Z
dc.identifier.issn1367-4803
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/288691
dc.description.abstractCRISPR/Cas9 system requires short guide RNAs (sgRNAs) to direct genome modification. Most currently available tools for sgRNA design operate only with standard reference genomes, and are best suited for small-scale projects. To address these limitations, we developed Crisflash, a software tool for fast sgRNA design and potential off-target discovery, built for performance and flexibility. Crisflash can rapidly design CRISPR guides against any sequenced genome or genome sequences, and can optimize guide accuracy by incorporating user-supplied variant data. Crisflash is over an order of magnitude faster than comparable tools, even using a single CPU core, and efficiently and robustly scores the potential off-targeting of all possible candidate CRISPR guide oligonucleotides.
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleCrisflash: Open-source software to generate CRISPR guide RNAs against genomes annotated with individual variationen
dc.typeArticle
prism.publicationNameOxford University Pressen
dc.identifier.doi10.17863/CAM.35954
dcterms.dateAccepted2019-01-07en
rioxxterms.versionofrecord10.1093/bioinformatics/btz019en
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2019-01-07en
dc.contributor.orcidOdom, Duncan [0000-0001-6201-5599]
dc.identifier.eissn1367-4811
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idCancer Research UK (C14303_do not transfer)
pubs.funder-project-idCancer Research UK (20412)
pubs.funder-project-idWellcome Trust (202878/Z/16/Z)
pubs.funder-project-idEuropean Research Council (615584)
cam.issuedOnline2019-01-12en


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International