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dc.contributor.authorMao, Shiqingen
dc.contributor.authorNguyen, Haen
dc.contributor.authorStewart, Sabrinaen
dc.contributor.authorKukwikila, Mikiemboen
dc.contributor.authorJones, Sioneden
dc.contributor.authorOffenbartl-Stiegert, Danielen
dc.contributor.authorBeck, Stephanen
dc.contributor.authorHoworka, Stefanen
dc.contributor.authorBalasubramanian, Shankaren
dc.date.accessioned2019-02-21T00:30:08Z
dc.date.available2019-02-21T00:30:08Z
dc.identifier.issn1521-3773
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/289737
dc.description.abstractControlling the functional dynamics of DNA within living cells is essential in biomedical research. Epigenetic modifications such as DNA methylation play a key role in this process. Controlled DNA methylation editing can be attained via genetic means. Yet there are few chemical tools available for the spatial and temporal modulation of this modification. Here we present a small-molecule approach to modulate DNA methylation with light. The strategy uses a photo-tuneable version of a clinically used drug (5-aza-2-deoxycytidine) to alter the catalytic activity of DNA methyltransferases, the enzymes that methylate DNA. After uptake by cells, the photo-regulated molecule can be light-controlled to reduce genome-wide DNA methylation levels in proliferating cells. The chemical tool complements genetic, biochemical and pharmacological approaches to study the role of DNA methylation in biology and medicine.
dc.description.sponsorshipBBSRC; EPSRC; Leverhulme Trust; RS Wolfson Research Merit Award; MRC
dc.publisherJohn Wiley & Sons Ltd.
dc.titleOpto-epigenetic modulation of DNA methylation with a photo-responsive small-molecule approachen
dc.typeArticle
prism.publicationNameAngewandte Chemie - International Editionen
dc.identifier.doi10.17863/CAM.36982
dcterms.dateAccepted2019-02-13en
rioxxterms.versionofrecord10.1002/anie.201901139en
rioxxterms.versionAM
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2019-02-13en
dc.contributor.orcidBalasubramanian, Shankar [0000-0002-0281-5815]
dc.identifier.eissn1521-3773
rioxxterms.typeJournal Article/Reviewen
pubs.funder-project-idWellcome Trust (099232/Z/12/Z)
pubs.funder-project-idCancer Research UK (CB4330)
pubs.funder-project-idWellcome Trust (209441/Z/17/Z)
cam.issuedOnline2019-02-17en
cam.orpheus.successThu Jan 30 10:50:30 GMT 2020 - Embargo updated*
rioxxterms.freetoread.startdate2020-02-17


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