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dc.contributor.authorRunwal, Gautam
dc.date.accessioned2019-03-04T16:00:43Z
dc.date.available2019-03-04T16:00:43Z
dc.date.issued2019-03-28
dc.date.submitted2018-09-25
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/290149
dc.description.abstractAutophagy is an evolutionarily conserved process across eukaryotes that is responsible for degradation of cargo such as aggregate-prone proteins, pathogens, damaged organelles, macromolecules etc. via its delivery to lysosomes. The process is known to involve the formation of a double-membraned structure, called autophagosome, that engulfs the cargo destined for degradation and delivers its contents by fusing with lysosomes. This process involves several proteins at its core which include two transmembrane proteins, ATG9 and VMP1. While ATG9 and VMP1 has been discovered for about a decade and half, the trafficking and function of these proteins remain relatively unclear. My work in this thesis identifies and characterises a novel trafficking route for ATG9 and VMP1 and shows that both these proteins traffic via the dynamin-independent ARF6-associated pathway. Moreover, I also show that these proteins physically interact with each other. In addition, the tools developed during these studies helped me identify a new role for the most common autophagy receptor protein, p62. I show that p62 can specifically associate with and sequester LC3-I in autophagy-impaired cells (ATG9 and ATG16 null cells) leading to formation of LC3-positive structures that can be misinterpreted as mature autophagosomes. Perturbations in the levels of p62 were seen to affect the formation of these LC3-positive structures in cells. This observation, therefore, questions the reliability of LC3-immunofluorescence assays in autophagy-impaired cells as method of assessing autophagy and points towards the homeostatic function played by p62 in autophagy-impaired cells.
dc.description.sponsorshipCommonwealth Scholarships Commission, UK
dc.language.isoen
dc.rightsAll rights reserved
dc.subjectATG9
dc.subjectp62
dc.subjectVMP1
dc.subjectAutophagy-deficient cells
dc.subjectAutophagy assay
dc.titleThe study of two transmembrane autophagy proteins and the autophagy receptor, p62
dc.typeThesis
dc.type.qualificationlevelDoctoral
dc.type.qualificationnameDoctor of Philosophy (PhD)
dc.publisher.institutionUniversity of Cambridge
dc.publisher.departmentCambridge Institute for Medical Research
dc.date.updated2019-03-04T12:03:27Z
dc.identifier.doi10.17863/CAM.37378
dc.publisher.collegeSt. John's College
dc.type.qualificationtitlePhD in Medical Genetics
cam.supervisorRubinsztein, David
cam.thesis.fundingfalse
rioxxterms.freetoread.startdate2020-03-04


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