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dc.contributor.authorTysoe, Olivia
dc.contributor.authorJustin, Alexander
dc.contributor.authorBrevini, Teresa
dc.contributor.authorChen, Si Emma
dc.contributor.authorMahbubani, Krishnaa
dc.contributor.authorFrank, Anna K
dc.contributor.authorZedira, Hajer
dc.contributor.authorMelum, Espen
dc.contributor.authorSaeb-Parsy, Kourosh
dc.contributor.authorMarkaki, Athina
dc.contributor.authorVallier, Ludovic
dc.contributor.authorSampaziotis, Fotios
dc.date.accessioned2019-06-17T23:30:54Z
dc.date.available2019-06-17T23:30:54Z
dc.date.issued2019-06
dc.identifier.issn1754-2189
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/293672
dc.description.abstractPediatric liver transplantation is often required as a consequence of biliary disorders because of the lack of alternative treatments for repairing or replacing damaged bile ducts. To address the lack of availability of pediatric livers suitable for transplantation, we developed a protocol for generating bioengineered biliary tissue suitable for biliary reconstruction. Our platform allows the derivation of cholangiocyte organoids (COs) expressing key biliary markers and retaining functions of primary extra- or intrahepatic duct cholangiocytes within 2 weeks of isolation. COs are subsequently seeded on polyglycolic acid (PGA) scaffolds or densified collagen constructs for 4 weeks to generate bioengineered tissue retaining biliary characteristics. Expertise in organoid culture and tissue engineering is desirable for optimal results. COs correspond to mature functional cholangiocytes, differentiating our method from alternative organoid systems currently available that propagate adult stem cells. Consequently, COs provide a unique platform for studies in biliary physiology and pathophysiology, and the resulting bioengineered tissue has broad applications for regenerative medicine and cholangiopathies.
dc.format.mediumPrint-Electronic
dc.languageeng
dc.publisherSpringer Science and Business Media LLC
dc.rightsAll rights reserved
dc.subjectBile Ducts
dc.subjectOrganoids
dc.subjectCells, Cultured
dc.subjectAnimals
dc.subjectHumans
dc.subjectMice
dc.subjectBiocompatible Materials
dc.subjectTissue Engineering
dc.subjectCell Separation
dc.subjectEquipment Design
dc.subjectRegeneration
dc.subjectTissue Scaffolds
dc.titleIsolation and propagation of primary human cholangiocyte organoids for the generation of bioengineered biliary tissue.
dc.typeArticle
prism.endingPage1925
prism.issueIdentifier6
prism.publicationDate2019
prism.publicationNameNat Protoc
prism.startingPage1884
prism.volume14
dc.identifier.doi10.17863/CAM.40784
dcterms.dateAccepted2019-03-19
rioxxterms.versionofrecord10.1038/s41596-019-0168-0
rioxxterms.versionAM
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2019-06
dc.contributor.orcidMahbubani, Krishnaa [0000-0002-1327-2334]
dc.contributor.orcidFrank, Anna K [0000-0003-1378-5129]
dc.contributor.orcidSaeb-Parsy, Kourosh [0000-0002-0633-3696]
dc.contributor.orcidMarkaki, Athina [0000-0002-2265-1256]
dc.contributor.orcidVallier, Ludovic [0000-0002-3848-2602]
dc.contributor.orcidSampaziotis, Fotios [0000-0003-0812-7586]
dc.identifier.eissn1750-2799
rioxxterms.typeJournal Article/Review
pubs.funder-project-idIsaac Newton Trust (18.07i(c))
pubs.funder-project-idEngineering and Physical Sciences Research Council (EP/N509620/1)
pubs.funder-project-idMRC (1621144)
pubs.funder-project-idMedical Research Council (MC_PC_12009)
pubs.funder-project-idAcademy of Medical Sciences (SGL019\1071)
pubs.funder-project-idEuropean Research Council (741707)
cam.issuedOnline2019-05-20
rioxxterms.freetoread.startdate2019-12-30


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