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dc.contributor.authorKumar, Narender
dc.contributor.authorPeacock, Sharon J
dc.date.accessioned2019-10-25T23:30:17Z
dc.date.available2019-10-25T23:30:17Z
dc.date.issued2019-11-29
dc.identifier.issn2045-2322
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/298085
dc.description.abstractAbstract The major human pathogen Mycobacterium tuberculosis is rarely reported to cause disease in other animals. Cases in livestock are thought to occur through contact with infected handlers, but previous studies evaluating putative livestock-human transmission used typing techniques with limited resolution. Here, we undertook cross-sectional surveillance for tuberculosis in 271 livestock handlers and 167 cattle on three farms in Chennai, India and defined the relatedness of cultured isolates using whole genome sequencing. Humans and livestock were screened for active mycobacterial infection, and opportunistic post-mortem examination was performed on comparative intradermal test-positive cattle that died. Four cattle and 6 handlers on two farms were culture-positive for M. tuberculosis; M. bovis was not isolated. All 10 isolates (one from each case) belonged to Lineage 1. Pairwise genome comparisons of single nucleotide polymorphism (SNP) differences ranged from 1 to 600 SNPs, but 3 isolate pairs were less than 5 SNPs different. Two pairs were from handlers and the third pair were from two cattle on the same farm. The minimum pairwise SNP difference between a cattle and human isolate was >250 SNPs. Our study confirms the presence of M. tuberculosis infection in cattle in India, sequencing of which characterised relatedness between human and cattle-derived isolates.
dc.description.sponsorshipThis work was supported by ICMR-NIRT intramural research funding and Science and Engineering Research Board (DST-SERB), UK Medical Research Council (MR/N501864/1) MRC Joint Centre Partnership) and the Department of Biotechnology, India ((BT/IN/DBT-MRC (UK)/12/SS/2015-2016 for ICMR-National Institute for Research in Tuberculosis) as a Cambridge Chennai Partnership on Antimicrobial Resistant Tuberculosis.
dc.publisherNature Publishing Group
dc.rightsAll rights reserved
dc.rightsAttribution 4.0 International (CC BY)
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.titleIsolation and comparative genomics of Mycobacterium tuberculosis isolates from cattle and their attendants in South India
dc.typeArticle
prism.publicationNameScientific Reports
dc.identifier.doi10.17863/CAM.45143
dcterms.dateAccepted2019-10-22
rioxxterms.versionofrecord10.1038/s41598-019-54268-x
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2019-10-22
dc.contributor.orcidPeacock, Sharon [0000-0002-1718-2782]
dc.identifier.eissn2045-2322
rioxxterms.typeJournal Article/Review
pubs.funder-project-idMedical Research Council (MR/N501864/1)
cam.issuedOnline2019-11-29
cam.orpheus.successThu Jan 30 10:36:36 GMT 2020 - The item has an open VoR version.
rioxxterms.freetoread.startdate2100-01-01


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