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dc.contributor.authorMund, Men
dc.contributor.authorBeek, Jen
dc.contributor.authorDeschamps, Jen
dc.contributor.authorDmitrieff, Sen
dc.contributor.authorHoess, Pen
dc.contributor.authorMonster, Jen
dc.contributor.authorPicco, Aen
dc.contributor.authorNedelec, Francoisen
dc.contributor.authorKaksonen, Men
dc.contributor.authorRies, Jen
dc.date.accessioned2020-02-21T00:30:06Z
dc.date.available2020-02-21T00:30:06Z
dc.date.issued2018-08-09en
dc.identifier.issn0092-8674
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/302444
dc.description.abstractClathrin-mediated endocytosis is an essential cellular function in all eukaryotes that is driven by a self-assembled macromolecular machine of over 50 different proteins in tens to hundreds of copies. How these proteins are organized to produce endocytic vesicles with high precision and efficiency is not understood. Here, we developed high-throughput superresolution microscopy to reconstruct the nanoscale structural organization of 23 endocytic proteins from over 100,000 endocytic sites in yeast. We found that proteins assemble by radially ordered recruitment according to function. WASP family proteins form a circular nanoscale template on the membrane to spatially control actin nucleation during vesicle formation. Mathematical modeling of actin polymerization showed that this WASP nano-template optimizes force generation for membrane invagination and substantially increases the efficiency of endocytosis. Such nanoscale pre-patterning of actin nucleation may represent a general design principle for directional force generation in membrane remodeling processes such as during cell migration and division.
dc.publisherElsevier
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleSystematic superresolution analysis of endocytosis reveals an actin nucleation nano-template that drives efficient vesicle formation.en
dc.typeArticle
prism.endingPage311
prism.issueIdentifier26en
prism.publicationDate2018en
prism.publicationNameCellen
prism.startingPage311
prism.volume29en
dc.identifier.doi10.17863/CAM.49515
dcterms.dateAccepted2018-06-13en
rioxxterms.versionofrecord10.1016/j.cell.2018.06.032en
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserveden
rioxxterms.licenseref.startdate2018-08-09en
dc.contributor.orcidNedelec, Francois [0000-0002-8141-5288]
dc.identifier.eissn1939-4586
rioxxterms.typeJournal Article/Reviewen
cam.issuedOnline2018-07-26en


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International