Show simple item record

dc.contributor.authorRheinlaender, Johannes
dc.contributor.authorDimitracopoulos, Andrea
dc.contributor.authorWallmeyer, Bernhard
dc.contributor.authorKronenberg, Nils M
dc.contributor.authorChalut, Kevin
dc.contributor.authorGather, Malte C
dc.contributor.authorBetz, Timo
dc.contributor.authorCharras, Guillaume
dc.contributor.authorFranze, Kristian
dc.date.accessioned2020-04-16T23:30:39Z
dc.date.available2020-04-16T23:30:39Z
dc.date.issued2020-09
dc.identifier.issn1476-1122
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/304442
dc.description.abstractCortical stiffness is an important cellular property that changes during migration, adhesion and growth. Previous atomic force microscopy (AFM) indentation measurements of cells cultured on deformable substrates have suggested that cells adapt their stiffness to that of their surroundings. Here we show that the force applied by AFM to a cell results in a significant deformation of the underlying substrate if this substrate is softer than the cell. This 'soft substrate effect' leads to an underestimation of a cell's elastic modulus when analysing data using a standard Hertz model, as confirmed by finite element modelling and AFM measurements of calibrated polyacrylamide beads, microglial cells and fibroblasts. To account for this substrate deformation, we developed a 'composite cell-substrate model'. Correcting for the substrate indentation revealed that cortical cell stiffness is largely independent of substrate mechanics, which has major implications for our interpretation of many physiological and pathological processes.
dc.format.mediumPrint-Electronic
dc.languageeng
dc.publisherSpringer Science and Business Media LLC
dc.rightsAll rights reserved
dc.subjectCerebral Cortex
dc.subjectMicroscopy, Atomic Force
dc.subjectCell Differentiation
dc.subjectSubstrate Specificity
dc.subjectElastic Modulus
dc.titleCortical cell stiffness is independent of substrate mechanics.
dc.typeArticle
prism.endingPage1025
prism.issueIdentifier9
prism.publicationDate2020
prism.publicationNameNat Mater
prism.startingPage1019
prism.volume19
dc.identifier.doi10.17863/CAM.51522
dcterms.dateAccepted2020-04-15
rioxxterms.versionofrecord10.1038/s41563-020-0684-x
rioxxterms.versionAM
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2020-09
dc.contributor.orcidRheinlaender, Johannes [0000-0002-1976-9245]
dc.contributor.orcidDimitracopoulos, Andrea [0000-0001-6776-4214]
dc.contributor.orcidKronenberg, Nils M [0000-0001-6386-3848]
dc.contributor.orcidChalut, Kevin [0000-0001-6200-9690]
dc.contributor.orcidGather, Malte C [0000-0002-4857-5562]
dc.contributor.orcidBetz, Timo [0000-0002-1548-0655]
dc.contributor.orcidCharras, Guillaume [0000-0002-7902-0279]
dc.contributor.orcidFranze, Kristian [0000-0002-8425-7297]
dc.identifier.eissn1476-4660
rioxxterms.typeJournal Article/Review
pubs.funder-project-idBiotechnology and Biological Sciences Research Council (BB/N006402/1)
pubs.funder-project-idEuropean Research Council (772426)
pubs.funder-project-idEuropean Research Council (772798)
pubs.funder-project-idMedical Research Council (MC_PC_12009)
pubs.funder-project-idMedical Research Council (MC_PC_17230)
cam.issuedOnline2020-05-25
cam.orpheus.successMon Jul 20 07:54:43 BST 2020 - Embargo updated
cam.orpheus.counter14
rioxxterms.freetoread.startdate2020-11-25


Files in this item

Thumbnail
Thumbnail

This item appears in the following Collection(s)

Show simple item record