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dc.contributor.authorSerres, Murielle P
dc.contributor.authorSamwer, Matthias
dc.contributor.authorTruong Quang, Binh An
dc.contributor.authorLavoie, Geneviève
dc.contributor.authorPerera, Upamali
dc.contributor.authorGörlich, Dirk
dc.contributor.authorCharras, Guillaume
dc.contributor.authorPetronczki, Mark
dc.contributor.authorRoux, Philippe P
dc.contributor.authorPaluch, Ewa K
dc.date.accessioned2020-07-02T23:30:52Z
dc.date.available2020-07-02T23:30:52Z
dc.date.issued2020-01-27
dc.identifier.issn1534-5807
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/307532
dc.description.abstractMost metazoan cells entering mitosis undergo characteristic rounding, which is important for accurate spindle positioning and chromosome separation. Rounding is driven by contractile tension generated by myosin motors in the sub-membranous actin cortex. Recent studies highlight that alongside myosin activity, cortical actin organization is a key regulator of cortex tension. Yet, how mitotic actin organization is controlled remains poorly understood. To address this, we characterized the F-actin interactome in spread interphase and round mitotic cells. Using super-resolution microscopy, we then screened for regulators of cortex architecture and identified the intermediate filament vimentin and the actin-vimentin linker plectin as unexpected candidates. We found that vimentin is recruited to the mitotic cortex in a plectin-dependent manner. We then showed that cortical vimentin controls actin network organization and mechanics in mitosis and is required for successful cell division in confinement. Together, our study highlights crucial interactions between cytoskeletal networks during cell division.
dc.format.mediumPrint-Electronic
dc.languageeng
dc.publisherElsevier BV
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectHela Cells
dc.subjectIntermediate Filaments
dc.subjectHumans
dc.subjectVimentin
dc.subjectActins
dc.subjectChromosome Segregation
dc.subjectMitosis
dc.subjectInterphase
dc.subjectCell Physiological Phenomena
dc.subjectActin Cytoskeleton
dc.titleF-Actin Interactome Reveals Vimentin as a Key Regulator of Actin Organization and Cell Mechanics in Mitosis.
dc.typeArticle
prism.endingPage222.e7
prism.issueIdentifier2
prism.publicationDate2020
prism.publicationNameDev Cell
prism.startingPage210
prism.volume52
dc.identifier.doi10.17863/CAM.54622
dcterms.dateAccepted2019-12-12
rioxxterms.versionofrecord10.1016/j.devcel.2019.12.011
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2020-01-09
dc.contributor.orcidPaluch, Ewa Kamila [0000-0003-4691-2323]
dc.identifier.eissn1878-1551
rioxxterms.typeJournal Article/Review
cam.issuedOnline2020-01-09


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Attribution-NonCommercial-NoDerivatives 4.0 International
Except where otherwise noted, this item's licence is described as Attribution-NonCommercial-NoDerivatives 4.0 International