Show simple item record

dc.contributor.authorLi, Zhu-Hong
dc.contributor.authorKing, Thayer P
dc.contributor.authorAyong, Lawrence
dc.contributor.authorAsady, Beejan
dc.contributor.authorCai, Xinjiang
dc.contributor.authorRahman, Md Taufiq
dc.contributor.authorVella, Stephen A
dc.contributor.authorCoppens, Isabelle
dc.contributor.authorPatel, Sandip
dc.contributor.authorMoreno, Silvia NJ
dc.date.accessioned2021-11-25T17:28:32Z
dc.date.available2021-11-25T17:28:32Z
dc.date.issued2021-10-04
dc.identifier.issn2041-1723
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/331172
dc.description.abstractTwo-pore channels (TPCs) are a ubiquitous family of cation channels that localize to acidic organelles in animals and plants to regulate numerous Ca2+-dependent events. Little is known about TPCs in unicellular organisms despite their ancient origins. Here, we characterize a TPC from Toxoplasma gondii, the causative agent of toxoplasmosis. TgTPC is a member of a novel clad of TPCs in Apicomplexa, distinct from previously identified TPCs and only present in coccidians. We show that TgTPC localizes not to acidic organelles but to the apicoplast, a non-photosynthetic plastid found in most apicomplexan parasites. Conditional silencing of TgTPC resulted in progressive loss of apicoplast integrity, severely affecting growth and the lytic cycle. Isolation of TPC null mutants revealed a selective role for TPCs in replication independent of apicoplast loss that required conserved residues within the pore-lining region. Using a genetically-encoded Ca2+ indicator targeted to the apicoplast, we show that Ca2+ signals deriving from the ER but not from the extracellular space are selectively transmitted to the lumen. Deletion of the TgTPC gene caused reduced apicoplast Ca2+ uptake and membrane contact site formation between the apicoplast and the ER. Fundamental roles for TPCs in maintaining organelle integrity, inter-organelle communication and growth emerge.
dc.format.mediumElectronic
dc.languageeng
dc.publisherSpringer Science and Business Media LLC
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleA plastid two-pore channel essential for inter-organelle communication and growth of Toxoplasma gondii.
dc.typeArticle
prism.issueIdentifier1
prism.publicationDate2021
prism.publicationNameNat Commun
prism.startingPage5802
prism.volume12
dc.identifier.doi10.17863/CAM.78619
dc.identifier.doi10.17863/CAM.78619
dcterms.dateAccepted2021-09-06
rioxxterms.versionofrecord10.1038/s41467-021-25987-5
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.licenseref.startdate2021-10-04
dc.contributor.orcidLi, Zhu-Hong [0000-0003-4940-0729]
dc.contributor.orcidCai, Xinjiang [0000-0001-8933-7133]
dc.contributor.orcidRahman, Md Taufiq [0000-0003-3830-5160]
dc.contributor.orcidVella, Stephen A [0000-0002-0626-8708]
dc.contributor.orcidPatel, Sandip [0000-0001-7247-2013]
dc.contributor.orcidMoreno, Silvia NJ [0000-0002-2041-6295]
dc.identifier.eissn2041-1723
rioxxterms.typeJournal Article/Review
cam.issuedOnline2021-10-04


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International