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dc.contributor.authorScherer, Katharina
dc.contributor.authorMascheroni, Luca
dc.contributor.authorCarnell, George
dc.contributor.authorWunderlich, Lucia
dc.contributor.authorMakarchuk, Stanislaw
dc.contributor.authorBrockhoff, Marius
dc.contributor.authorMela, Ioanna
dc.contributor.authorFernandez-Villegas, Ana
dc.contributor.authorBarysevich, Max
dc.contributor.authorShaw, Hazel
dc.contributor.authorSans, Maria Suau
dc.contributor.authorGeorge, Charlotte
dc.contributor.authorLamb, Jacob
dc.contributor.authorKaminski-Schierle, Gabriele
dc.contributor.authorHeeney, Jonathan
dc.contributor.authorKaminski, Clemens
dc.date.accessioned2022-01-12T00:30:05Z
dc.date.available2022-01-12T00:30:05Z
dc.date.issued2022-01
dc.identifier.issn2375-2548
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/332628
dc.description.abstractDespite being the target of extensive research efforts due to the COVID-19 pandemic, relatively little is known about the dynamics of SARS-CoV-2 replication within cells. We investigate and characterise the tightly orchestrated sequence of events during different stages of the infection cycle by visualising the spatiotemporal dynamics of the four structural proteins of SARS-CoV-2 at high resolution. The nucleoprotein is expressed first and accumulates around folded ER membranes in convoluted layers that connect to viral RNA replication foci. We find that of the three transmembrane proteins, the membrane protein appears at the Golgi apparatus/ERGIC before the spike and envelope proteins. Relocation of the lysosome marker LAMP1 towards the assembly compartment and its detection in transport vesicles of viral proteins confirm an important role of lysosomes in SARS-CoV-2 egress. These data provide new insights into the spatiotemporal regulation of SARS-CoV-2 assembly, and refine current understanding of SARS-CoV-2 replication.
dc.description.sponsorshipAstraZeneca; Infinitus (China) Ltd.
dc.publisherAmerican Association for the Advancement of Science
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.titleSARS-CoV-2 nucleocapsid protein adheres to replication organelles before viral assembly at the Golgi/ERGIC and lysosome-mediated egress
dc.typeArticle
prism.publicationDate2021
prism.publicationNameScience Advances
dc.identifier.doi10.17863/CAM.80073
dcterms.dateAccepted2021-11-01
rioxxterms.versionofrecord10.1126/sciadv.abl4895
rioxxterms.versionVoR
rioxxterms.licenseref.urihttp://creativecommons.org/licenses/by/4.0/
rioxxterms.licenseref.startdate2021-06-15
dc.contributor.orcidScherer, Katharina [0000-0002-2042-5407]
dc.contributor.orcidCarnell, George [0000-0001-8875-0989]
dc.contributor.orcidMakarchuk, Stanislaw [0000-0001-5484-7141]
dc.contributor.orcidMela, Ioanna [0000-0002-2914-9971]
dc.contributor.orcidShaw, Hazel [0000-0003-1438-1089]
dc.contributor.orcidHeeney, Jonathan [0000-0003-2702-1621]
dc.contributor.orcidKaminski, Clemens [0000-0002-5194-0962]
dc.identifier.eissn2375-2548
rioxxterms.typeJournal Article/Review
pubs.funder-project-idEngineering and Physical Sciences Research Council (EP/H018301/1)
pubs.funder-project-idWellcome Trust (089703/Z/09/Z)
pubs.funder-project-idMedical Research Council (MR/K015850/1)
pubs.funder-project-idMedical Research Council (MR/K02292X/1)
pubs.funder-project-idEngineering and Physical Sciences Research Council (EP/L015889/1)
pubs.funder-project-idMedical Research Council (G0902243)
cam.issuedOnline2022-01-07
datacite.issupplementedby.urlhttps://doi.org/10.17863/CAM.78118


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Attribution 4.0 International
Except where otherwise noted, this item's licence is described as Attribution 4.0 International