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dc.contributor.authorWang, Limin
dc.contributor.authorNing, Youzheng
dc.contributor.authorSun, Jian
dc.contributor.authorWilkins, Katie A
dc.contributor.authorMatthus, Elsa
dc.contributor.authorMcNelly, Rose E
dc.contributor.authorDark, Adeeba
dc.contributor.authorRubio, Lourdes
dc.contributor.authorMoeder, Wolfgang
dc.contributor.authorYoshioka, Keiko
dc.contributor.authorVéry, Anne-Aliénor
dc.contributor.authorStacey, Gary
dc.contributor.authorLeblanc-Fournier, Nathalie
dc.contributor.authorLegué, Valérie
dc.contributor.authorMoulia, Bruno
dc.contributor.authorDavies, Julia
dc.date.accessioned2022-01-25T00:31:00Z
dc.date.available2022-01-25T00:31:00Z
dc.date.issued2022-01-25
dc.identifier.issn0028-646X
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/332903
dc.description.abstractDamage can be signalled by extracellular ATP (eATP) using plasma membrane (PM) receptors to effect cytosolic free Ca2+ ([Ca2+ ]cyt ) increase as a second messenger. The downstream PM Ca2+ channels remain enigmatic. Here, the Arabidopsis thaliana Ca2+ channel subunit Cyclic Nucleotide-Gated Channel2 (CNGC2) was identified as a critical component linking eATP receptors to downstream [Ca2+ ]cyt signalling in roots. eATP-induced changes in single epidermal cell PM voltage and conductance were measured electrophysiologically, changes in root [Ca2+ ]cyt were measured with aequorin and root transcriptional changes were determined by qRT-PCR. Two cngc2 loss of function mutants were used: cngc2-3 and dnd1 (which expresses cytosolic aequorin). eATP-induced transient depolarisation of Arabidopsis root elongation zone epidermal PM voltage was Ca2+ -dependent, requiring CNGC2 but not CNGC4 (its channel co-subunit in immunity signalling). Activation of PM Ca2+ influx currents also required CNGC2. The eATP-induced [Ca2+ ]cyt increase and transcriptional response in cngc2 roots were significantly impaired. CNGC2 is required for eATP-induced epidermal Ca2+ influx, causing depolarisation leading to [Ca2+ ]cyt increase and damage-related transcriptional response.
dc.publisherWiley
dc.rightsAll Rights Reserved
dc.rights.urihttp://www.rioxx.net/licenses/all-rights-reserved
dc.titleArabidopsis thaliana Cyclic Nucleotide-Gated Channel2 mediates extracellular ATP signal transduction in root epidermis.
dc.typeArticle
dc.publisher.departmentDepartment of Plant Sciences
dc.date.updated2022-01-24T10:35:27Z
prism.publicationNameNew Phytol
dc.identifier.doi10.17863/CAM.80333
dcterms.dateAccepted2022-01-16
rioxxterms.versionofrecord10.1111/nph.17987
rioxxterms.versionAM
dc.contributor.orcidRubio, Lourdes [0000-0002-7747-2722]
dc.contributor.orcidYoshioka, Keiko [0000-0002-3797-4277]
dc.contributor.orcidDavies, Julia [0000-0003-2630-4339]
dc.identifier.eissn1469-8137
rioxxterms.typeJournal Article/Review
pubs.funder-project-idBiotechnology and Biological Sciences Research Council (BB/S004637/1)
cam.issuedOnline2022-01-25
cam.orpheus.successTue Feb 01 19:02:46 GMT 2022 - Embargo updated*
cam.depositDate2022-01-24
pubs.licence-identifierapollo-deposit-licence-2-1
pubs.licence-display-nameApollo Repository Deposit Licence Agreement
rioxxterms.freetoread.startdate2023-01-25


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