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dc.contributor.authorChorro, Adrià
dc.contributor.authorVerma, Bhavna
dc.contributor.authorHomfeldt, Maylin
dc.contributor.authorIbáñez, Beatríz
dc.contributor.authorLawrence, Peter A
dc.contributor.authorCasal Jimenez, Jose
dc.date.accessioned2022-06-24T11:56:27Z
dc.date.available2022-06-24T11:56:27Z
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/338338
dc.descriptionThe slope of a supracellular molecular gradient has long been thought to orient and coordinate planar cell polarity (PCP). Here we demonstrate and measure that gradient. Dachsous (Ds) is a conserved and elemental molecule of PCP; Ds forms intercellular bridges with another cadherin molecule, Fat (Ft), an interaction modulated by the Golgi protein Four-jointed (Fj). Using genetic mosaics and tagged Ds we measure Ds in vivo in membranes of individual cells over a whole metamere of the Drosophila abdomen. We find: 1. A supracellular gradient that rises from head to tail in the anterior compartment (A) and then falls in the posterior compartment (P). 2. There is more Ds in the front than the rear membranes of all cells in the A compartment, except that compartment's most anterior and most posterior cells. There is more Ds in the rear than in the front membranes of all cells of the P compartment 3. The loss of Fj removes intracellular asymmetry anteriorly in the segment and reduces it elsewhere. Additional experiments show that Fj makes PCP more robust. Using Dachs (D) as a molecular indicator of polarity, we confirm that opposing gradients of PCP meet slightly out of register with compartment boundaries. Please see readme document for further details
dc.description.sponsorshipWellcome Trust (107060/Z/15/Z)
dc.formatImageJ (Fiji), Microsoft Excel, RStudio
dc.rightsAttribution 4.0 International (CC BY 4.0)
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectDrosophila, Planar Cell Polarity
dc.titleResearch data supporting "Planar cell polarity: intracellular asymmetry and supracellular gradients of Dachsous"
dc.typeDataset
dc.publisher.departmentDepartment of Zoology
dc.date.updated2022-06-24T10:55:49Z
dc.identifier.doi10.17863/CAM.85748
rioxxterms.licenseref.urihttps://creativecommons.org/licenses/by/4.0/
datacite.contributor.supervisorLawrence, Peter A
dcterms.format*.txt (simple text), *.ijm (ImageJ Macro language), *.R (R script), *.xlsx, (Excel spreadsheet), *.xlsm (Excel macro-enabled spreadsheet)
dc.contributor.orcidCasal Jimenez, Jose [0000-0002-5149-1335]
rioxxterms.typeOther
pubs.funder-project-idWellcome Trust (107060/Z/15/Z)
datacite.issupplementto.doi10.1101/2022.06.18.496667
datacite.issupplementto.urlhttps://www.repository.cam.ac.uk/handle/1810/341969
cam.depositDate2022-06-24
pubs.licence-identifierapollo-deposit-licence-2-1
pubs.licence-display-nameApollo Repository Deposit Licence Agreement


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Attribution 4.0 International (CC BY 4.0)
Except where otherwise noted, this item's licence is described as Attribution 4.0 International (CC BY 4.0)