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dc.contributor.authorZhai, Qiaocheng
dc.contributor.authorZeng, Yizhun
dc.contributor.authorGu, Yue
dc.contributor.authorLi, Zhihao
dc.contributor.authorZhang, Tao
dc.contributor.authorYuan, Baoshi
dc.contributor.authorWang, Tao
dc.contributor.authorYan, Jie
dc.contributor.authorQin, Han
dc.contributor.authorYang, Ling
dc.contributor.authorChen, Xiaowei
dc.contributor.authorVidal-Puig, Antonio
dc.contributor.authorXu, Ying
dc.date.accessioned2022-07-04T23:30:20Z
dc.date.available2022-07-04T23:30:20Z
dc.date.issued2022-05-20
dc.identifier.issn2589-0042
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/338747
dc.description.abstractThe suprachiasmatic nucleus (SCN) integrates light and systemic signals from peripheral tissues to coordinate physiology and behavior daily rhythms. However, the contribution that nutrients and feeding patterns provide to the SCN network regulation remains controversial. Here, we found that time-restricted feeding (TRF) in ZT0-4 (Zeitgeber Time) generates a robust and long-term shift in locomotor behavior and increased wakefulness. Intracellular Ca2+ signals in SCN GABAergic neurons of freely moving mice showed significant activation after ZT0-4 TRF treatment. Furthermore, RNA-seq profiling of SCN showed that TRF during ZT0-4 increased Insulin-like Growth Factor 2 (Igf2) expression and dysregulated ion transporters, including the downregulation of Kcc2. SCN neuron-specific loss of function of Kcc2 amplified ZT0-4 TRF induced aftereffect. Moreover, overexpression of IGF2 in SCN GABAergic neurons extended the locomotion range, mirroring the TRF aftereffect. In summary, our study showed that the IGF2-KCC2 pathway plays an important role for TRF induced behavior changes.
dc.format.mediumElectronic-eCollection
dc.publisherElsevier BV
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subjectBehavioral neuroscience
dc.subjectMolecular neuroscience
dc.subjectNeuroscience
dc.titleTime-restricted feeding entrains long-term behavioral changes through the IGF2-KCC2 pathway.
dc.typeArticle
dc.publisher.departmentDepartment of Clinical Biochemistry
dc.date.updated2022-07-01T14:50:52Z
prism.issueIdentifier5
prism.number104267
prism.publicationDate2022
prism.publicationNameiScience
prism.startingPage104267
prism.volume25
dc.identifier.doi10.17863/CAM.86157
dcterms.dateAccepted2022-04-13
rioxxterms.versionofrecord10.1016/j.isci.2022.104267
rioxxterms.versionVoR
dc.contributor.orcidVidal-Puig, Antonio [0000-0003-4220-9577]
dc.identifier.eissn2589-0042
rioxxterms.typeJournal Article/Review
cam.issuedOnline2022-04-18
cam.depositDate2022-07-01
pubs.licence-identifierapollo-deposit-licence-2-1
pubs.licence-display-nameApollo Repository Deposit Licence Agreement


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Attribution-NonCommercial-NoDerivatives 4.0 International
Except where otherwise noted, this item's licence is described as Attribution-NonCommercial-NoDerivatives 4.0 International