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Single-cell RNA-seq reveals hidden transcriptional variation in malaria parasites.

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cam.issuedOnline2018-03-27
dc.contributor.authorReid, Adam J
dc.contributor.authorTalman, Arthur M
dc.contributor.authorBennett, Hayley M
dc.contributor.authorGomes, Ana R
dc.contributor.authorSanders, Mandy J
dc.contributor.authorIllingworth, Christopher JR
dc.contributor.authorBillker, Oliver
dc.contributor.authorBerriman, Matthew
dc.contributor.authorLawniczak, Mara Kn
dc.contributor.orcidIllingworth, Christopher JR [0000-0002-0030-2784]
dc.contributor.orcidBillker, Oliver [0000-0003-1716-168X]
dc.contributor.orcidBerriman, Matthew [0000-0002-9581-0377]
dc.contributor.orcidLawniczak, Mara Kn [0000-0002-3006-2080]
dc.date.accessioned2018-10-10T05:16:49Z
dc.date.available2018-10-10T05:16:49Z
dc.date.issued2018-03-27
dc.description.abstractSingle-cell RNA-sequencing is revolutionising our understanding of seemingly homogeneous cell populations but has not yet been widely applied to single-celled organisms. Transcriptional variation in unicellular malaria parasites from the Plasmodium genus is associated with critical phenotypes including red blood cell invasion and immune evasion, yet transcriptional variation at an individual parasite level has not been examined in depth. Here, we describe the adaptation of a single-cell RNA-sequencing (scRNA-seq) protocol to deconvolute transcriptional variation for more than 500 individual parasites of both rodent and human malaria comprising asexual and sexual life-cycle stages. We uncover previously hidden discrete transcriptional signatures during the pathogenic part of the life cycle, suggesting that expression over development is not as continuous as commonly thought. In transmission stages, we find novel, sex-specific roles for differential expression of contingency gene families that are usually associated with immune evasion and pathogenesis.
dc.format.mediumElectronic
dc.identifier.doi10.17863/CAM.30721
dc.identifier.eissn2050-084X
dc.identifier.issn2050-084X
dc.identifier.urihttps://www.repository.cam.ac.uk/handle/1810/283353
dc.languageeng
dc.language.isoeng
dc.publishereLife Sciences Publications, Ltd
dc.publisher.urlhttp://dx.doi.org/10.7554/elife.33105
dc.rightsAttribution 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/
dc.subjectP. falciparum
dc.subjectcell biology
dc.subjectevolutionary biology
dc.subjectgenomics
dc.subjectmalaria
dc.subjectscRNAseq
dc.subjecttransmission
dc.subjectAnimals
dc.subjectGene Expression Profiling
dc.subjectGenetic Variation
dc.subjectHumans
dc.subjectPlasmodium
dc.subjectRodentia
dc.subjectSequence Analysis, RNA
dc.subjectSingle-Cell Analysis
dc.titleSingle-cell RNA-seq reveals hidden transcriptional variation in malaria parasites.
dc.typeArticle
dcterms.dateAccepted2018-03-04
prism.publicationDate2018
prism.publicationNameElife
prism.volume7
pubs.funder-project-idWellcome Trust (101239/Z/13/Z)
rioxxterms.licenseref.startdate2018-03-27
rioxxterms.licenseref.urihttp://www.rioxx.net/licenses/all-rights-reserved
rioxxterms.typeJournal Article/Review
rioxxterms.versionVoR
rioxxterms.versionofrecord10.7554/eLife.33105

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