Hyperpolarised 13C-MRI identifies the emergence of a glycolytic cell population within intermediate-risk human prostate cancer
cam.depositDate | 2021-12-06 | |
cam.orpheus.counter | 27 | * |
cam.orpheus.success | VoR added. | |
dc.contributor.author | Sushentsev, Nikita | |
dc.contributor.author | McLean, Mary A | |
dc.contributor.author | Warren, Anne | |
dc.contributor.author | Benjamin, Arnold JV | |
dc.contributor.author | Brodie, Cara | |
dc.contributor.author | Frary, Amy | |
dc.contributor.author | Gill, Andrew | |
dc.contributor.author | Jones, Julia | |
dc.contributor.author | Kaggie, Joshua | |
dc.contributor.author | Lamb, Benjamin W | |
dc.contributor.author | Locke, Matthew J | |
dc.contributor.author | Miller, Jodi L | |
dc.contributor.author | Mills, Ian G | |
dc.contributor.author | Priest, Andrew N | |
dc.contributor.author | Robb, Fraser JL | |
dc.contributor.author | Shah, Nimish | |
dc.contributor.author | Schulte, Rolf S | |
dc.contributor.author | Graves, Martin J | |
dc.contributor.author | Gnanapragasam, Vincent | |
dc.contributor.author | Brindle, Kevin | |
dc.contributor.author | Barrett, Tristan | |
dc.contributor.author | Gallagher, Ferdia | |
dc.contributor.orcid | Warren, Anne [0000-0002-1170-7867] | |
dc.contributor.orcid | Frary, Amy [0000-0002-4373-3517] | |
dc.contributor.orcid | Gill, Andrew [0000-0002-9287-9563] | |
dc.contributor.orcid | Kaggie, Joshua [0000-0001-6706-3442] | |
dc.contributor.orcid | Gnanapragasam, Vincent [0000-0003-4722-4207] | |
dc.contributor.orcid | Brindle, Kevin [0000-0003-3883-6287] | |
dc.contributor.orcid | Barrett, Tristan [0000-0002-1180-1474] | |
dc.contributor.orcid | Gallagher, Ferdia [0000-0003-4784-5230] | |
dc.date.accessioned | 2021-12-08T00:30:16Z | |
dc.date.available | 2021-12-08T00:30:16Z | |
dc.date.updated | 2021-12-06T09:00:30Z | |
dc.description.abstract | Hyperpolarised magnetic resonance imaging (HP-13C-MRI) is an emerging clinical technique to detect [1-13C]lactate production in prostate cancer (PCa) following intravenous injection of hyperpolarised [1-13C]pyruvate. Here we differentiate clinically-significant PCa from indolent disease in a low/intermediate-risk population by correlating [1-13C]lactate labelling on MRI with the percentage of Gleason pattern 4 (%GP4) disease. Using immunohistochemistry and spatial transcriptomics, we show that HP-13C-MRI predominantly measures metabolism in the epithelial compartment of the tumour, rather than the stroma. MRI-derived tumour [1-13C]lactate labelling correlated with epithelial mRNA expression of the enzyme lactate dehydrogenase (LDHA and LDHB combined), and the ratio of lactate transporter expression between the epithelial and stromal compartments (epithelium-to-stroma MCT4). We observe similar changes in MCT4, LDHA, and LDHB between tumours with primary Gleason patterns 3 and 4 in an independent TCGA cohort. Therefore, HP-13C-MRI can metabolically phenotype clinically significant disease based on underlying metabolic differences in the epithelial and stromal tumour compartments. | |
dc.description.sponsorship | Prostate Cancer UK (PCUK; Grant PA14-012) and Cancer Research UK (CRUK; Grants C19212/A27150, C19212/A16628). | |
dc.identifier.doi | 10.17863/CAM.78706 | |
dc.identifier.issn | 2041-1723 | |
dc.identifier.uri | https://www.repository.cam.ac.uk/handle/1810/331260 | |
dc.language.iso | eng | |
dc.publisher | Nature Research | |
dc.publisher.department | Department of Radiology | |
dc.publisher.department | Department of Biochemistry | |
dc.rights | Attribution 4.0 International | |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | |
dc.title | Hyperpolarised 13C-MRI identifies the emergence of a glycolytic cell population within intermediate-risk human prostate cancer | |
dc.type | Article | |
dcterms.dateAccepted | 2021-12-02 | |
prism.publicationName | Nature Communications | |
pubs.licence-display-name | Apollo Repository Deposit Licence Agreement | |
pubs.licence-identifier | apollo-deposit-licence-2-1 | |
rioxxterms.type | Journal Article/Review | |
rioxxterms.version | VoR |
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